Programa de Pós-Graduação Em Farmácia (PPGFar), Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Departamento de Análises Clínicas-CCS, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-970, Brazil.
Inflamm Res. 2022 Aug;71(7-8):741-758. doi: 10.1007/s00011-022-01584-0. Epub 2022 May 25.
Several experimental models have been designed to promote the development of new anti-inflammatory drugs. The in vitro model using RAW 264.7 cells has been widely used. However, there is still no consensus on which inflammatory mediators should initially be measured to screen for possible anti-inflammatory effects. To determine the rationality of measuring inflammatory mediators together with NO, such as the levels of tumor necrosis factor (TNF)-α, and interleukins (IL) 1β and 6, we carried out this systematic review (SR) and meta-analysis (MA).
We conducted this SR and MA in accordance with the Preferred Reporting of Systematic Reviews and Meta-Analysis and the Cochrane Handbook for Systematic Reviews of Intervention. This review was registered in the Open Science Framework ( https://doi.org/10.17605/OSF.IO/8C3HT ).
LPS-induced cells produced high NO levels compared to non-LPS induced, and this production was not related to cell density. TNF-α, IL-1β, and IL-6, also showed high levels after cells had been stimulated with LPS. Though with some restrictions, all studies were reliable, as the risk of bias was detected in the test compounds and systems.
Measurement of NO levels may be sufficient to screen for possible anti-inflammatory action in the context of LPS-induced RAW 264.7 cells.
已经设计了几种实验模型来促进新的抗炎药物的开发。使用 RAW 264.7 细胞的体外模型已被广泛应用。然而，对于应该首先测量哪些炎症介质来筛选可能的抗炎作用，目前仍没有共识。为了确定与 NO 一起测量炎症介质（如肿瘤坏死因子 (TNF)-α 和白细胞介素 (IL) 1β 和 6 的水平）的合理性，我们进行了这项系统评价 (SR) 和荟萃分析 (MA)。
我们根据系统评价和荟萃分析的首选报告和干预措施的 Cochrane 手册进行了这项 SR 和 MA。该综述已在开放科学框架 (https://doi.org/10.17605/OSF.IO/8C3HT) 中注册。
与非 LPS 诱导的细胞相比，LPS 诱导的细胞产生了高水平的 NO，并且这种产生与细胞密度无关。用 LPS 刺激细胞后，TNF-α、IL-1β 和 IL-6 也表现出高水平。尽管存在一些限制，但所有研究都是可靠的，因为在测试化合物和系统中检测到偏倚风险。
在 LPS 诱导的 RAW 264.7 细胞中，测量 NO 水平可能足以筛选可能的抗炎作用。
