Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea.
Research Institute, WithBio Inc., Seoul 05029, Republic of Korea.
J Microbiol Biotechnol. 2024 Feb 28;34(2):358-366. doi: 10.4014/jmb.2309.09007. Epub 2023 Oct 5.
The lactic acid bacteria, including and , have been widely studied for their preventive and therapeutic effects. In this study, the underlying mechanism of action for the antioxidant and immunostimulatory effects of two strains of heat-treated paraprobiotics was examined. Heat-treated KU15041 and KU15003 showed higher radical scavenging activity in both the 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays than the commercial probiotic strain LGG. In addition, treatment with these two strains exhibited immunostimulatory effects in RAW 264.7 macrophages, with KU15003 showing a slightly higher effect. Additionally, they promoted phagocytosis and NO production in RAW 264.7 cells without any cytotoxicity. Moreover, the expression of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 was upregulated. These strains resulted in an increased expression of inducible nitric oxide synthase and cyclooxygenase-2. Moreover, the nuclear factor-κB and mitogen-activated protein kinase signaling pathways were stimulated by these strains. These findings suggest the potential of using KU15041 and KU15003 in food or by themselves as probiotics with antioxidant and immune-enhancing properties.
乳酸菌，包括 和 ，因其预防和治疗作用而被广泛研究。在这项研究中，研究了两种热处理益生菌的抗氧化和免疫刺激作用的潜在作用机制。热处理的 KU15041 和 KU15003 在 2-氮杂双（3-乙基苯并噻唑啉-6-磺酸（ABTS）和 2,2-二苯基-1-苦基肼（DPPH）测定中均显示出比商业益生菌菌株 LGG 更高的自由基清除活性。此外，这两种菌株在 RAW 264.7 巨噬细胞中表现出免疫刺激作用，其中 KU15003 的作用略高。此外，它们在没有任何细胞毒性的情况下促进 RAW 264.7 细胞的吞噬作用和 NO 产生。此外，肿瘤坏死因子-α、白细胞介素（IL）-1β 和 IL-6 的表达上调。这些菌株导致诱导型一氧化氮合酶和环氧化酶-2 的表达增加。此外，这些菌株还刺激核因子-κB 和丝裂原激活蛋白激酶信号通路。这些发现表明，使用 KU15041 和 KU15003 作为具有抗氧化和免疫增强特性的食品或益生菌本身具有潜力。
