Qu Pengyu, He Lina, Xue Lulu, Liu Han, Li Xiaona, Zhao Huanhuan, Fu Liuyang, Han Suoyi, Dai Xiaodong, Dong Wenzhao, Shi Lei, Zhang Xinyou
School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.
Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement/National and Provincial Joint Engineering Laboratory for Peanut Genetic Improvement/The Shennong Laboratory/National Invocation Center for Bio-Breeding Industry, Zhengzhou 450002, China.
Int J Mol Sci. 2025 Jul 26;26(15):7243. doi: 10.3390/ijms26157243.
Peanut ( L.) is a globally important oilseed cash crop, yet its limited genetic diversity and unique reproductive biology present persistent challenges for conventional crossbreeding. Traditional breeding approaches are often time-consuming and inadequate, mitigating the pace of cultivar development. Essential for double fertilization and programmed cell death (PCD), DUF679 membrane proteins (DMPs) represent a membrane protein family unique to plants. In the present study, a comprehensive analysis of the DMP gene family in peanuts was conducted, which included the identification of 21 family members. Based on phylogenetic analysis, these genes were segregated into five distinct clades (I-V), with , , , and in clade IV exhibiting high homology with known haploid induction genes. These four candidates also displayed significantly elevated expression in floral tissues compared to other organs, supporting their candidacy for haploid induction in peanuts. Subcellular localization prediction, confirmed through co-localization assays, demonstrated that AhDMPs primarily localize to the plasma membrane, consistent with their proposed roles in the reproductive signaling process. Furthermore, chromosomal mapping and synteny analyses revealed that the expansion of the gene family is largely driven by whole-genome duplication (WGD) and segmental duplication events, reflecting the evolutionary dynamics of the tetraploid peanut genome. Collectively, these findings establish a foundational understanding of the gene family and highlight promising targets for future applications in haploid induction-based breeding strategies in peanuts.
花生（Arachis hypogaea L.）是一种全球重要的油料经济作物，然而其有限的遗传多样性和独特的生殖生物学特性给传统杂交育种带来了持续挑战。传统育种方法往往耗时且效果不佳，减缓了品种培育的速度。DUF679膜蛋白（DMPs）对于双受精和程序性细胞死亡（PCD）至关重要，代表了植物特有的一个膜蛋白家族。在本研究中，对花生中的DMP基因家族进行了全面分析，共鉴定出21个家族成员。基于系统发育分析，这些基因被分为五个不同的进化枝（I-V），其中进化枝IV中的AhDMP1、AhDMP2、AhDMP3和AhDMP4与已知的单倍体诱导基因具有高度同源性。这四个候选基因在花组织中的表达也显著高于其他器官，支持了它们在花生单倍体诱导中的候选地位。通过共定位分析证实的亚细胞定位预测表明，AhDMPs主要定位于质膜，与其在生殖信号传导过程中的作用一致。此外，染色体定位和共线性分析表明，DMP基因家族的扩张主要由全基因组复制（WGD）和片段重复事件驱动，反映了四倍体花生基因组的进化动态。这些发现共同建立了对DMP基因家族的基本认识，并突出了其在基于单倍体诱导的花生育种策略未来应用中的潜在靶点。
