Yu Lixia, Zhan Hui, Chu Caihua, Jin Diankun, Li Lingfeng, Li Juan, Wang Changming, Shupe Todd F, Wang Shuguang
Biological Research and Utilization Innovation Team in Bamboo Resources of Yunnan Province, Southwest Forestry University, Kunming, China.
Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, China.
Plant J. 2025 Aug;123(3):e70409. doi: 10.1111/tpj.70409.
Adventitious root (AR) growth is vital for mass propagation of bamboo, and there is a significant difference in the rooting ability among different bamboo species. The differences of AR differentiation from the bamboo branch were undefined. In this study, it was found that the thickness and lignification degrees of the cortex were closely related to the degree of rooting difficulty of the branch base in different bamboo species. The inner cortical cells restored their division ability and further differentiated AR primordium in Dendrocalamus brandisii. Indole-3-acetic acid (IAA) and jasmonic acid (JA) played a crucial role in the development of ARs, and further IAA and JA treatment analysis indicated that only low concentration hormones could enhance AR differentiation. DbCRL1 and DbAOS1 were defined to be related to the AR differentiation, and their positive functions were validated in rice. DbAOS1 exhibited an ability to independently enhance and activate ANTHRANILATE SYNTHASE in the auxin production pathway. DbCRL1 could interact with DbWOX11 in vivo and in vitro, wherein they promote AR differentiation synergistically. These findings revealed the differences of AR differentiation in the branch and provided new insights into the rooting mechanism of D. brandisii branch.
不定根（AR）生长对竹子的大规模繁殖至关重要，不同竹种间的生根能力存在显著差异。竹子枝条不定根分化的差异尚不明确。本研究发现，不同竹种中皮层的厚度和木质化程度与枝条基部生根难易程度密切相关。在勃氏甜龙竹中，皮层内部细胞恢复其分裂能力并进一步分化为不定根原基。吲哚 - 3 - 乙酸（IAA）和茉莉酸（JA）在不定根发育中起关键作用，进一步的IAA和JA处理分析表明，只有低浓度激素能增强不定根分化。DbCRL1和DbAOS1被确定与不定根分化有关，它们在水稻中的正向功能得到验证。DbAOS1具有在生长素合成途径中独立增强和激活邻氨基苯甲酸合酶的能力。DbCRL1在体内和体外均可与DbWOX11相互作用，二者协同促进不定根分化。这些发现揭示了枝条不定根分化的差异，并为勃氏甜龙竹枝条生根机制提供了新见解。
