Rys Magdalena, Bocianowski Jan, Dziurka Michał, Jurczyk Barbara, Stachurska Julia, Waligórski Piotr, Janeczko Anna
The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland.
Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland.
Int J Mol Sci. 2025 Aug 1;26(15):7408. doi: 10.3390/ijms26157408.
Global climate change is causing increasing fluctuations in winter temperatures, including episodes of warm conditions above 9 °C. Such events disrupt cold acclimation in plants and can induce deacclimation, reducing frost tolerance and altering, among other things, hormonal regulation. This study investigated hormonal and molecular changes associated with cold acclimation and deacclimation in oilseed rape ( L.) cultivars Kuga and Thure. Plants were grown under different conditions: non-acclimated (17 °C for three weeks), cold-acclimated (4 °C for three weeks), and deacclimated (16/9 °C day/night for one week). Detailed hormone analysis included auxins, gibberellins, cytokinins, stress-related hormones, and the expression of hormone-related genes (, , , , , , and ). Hormone concentrations in leaves changed dynamically in response to deacclimation with increased amounts of growth-promoting hormones and decreased amounts of stress hormones. Additionally, alterations in gene expression during deacclimation, such as in and , may function as protective mechanisms to help maintain or regain frost tolerance during reacclimation when temperatures decline again after the warm period. These findings improve the understanding of hormonal and molecular responses involved in the deacclimation of oilseed rape.
全球气候变化正导致冬季气温波动加剧，包括出现高于9摄氏度的温暖天气情况。此类事件会扰乱植物的冷驯化过程，并可能引发去驯化，降低抗冻性，还会改变激素调节等。本研究调查了油菜品种Kuga和Thure中与冷驯化和去驯化相关的激素及分子变化。植株在不同条件下生长：未驯化（17摄氏度，持续三周）、冷驯化（4摄氏度，持续三周）以及去驯化（白天16摄氏度/夜间9摄氏度，持续一周）。详细的激素分析包括生长素、赤霉素、细胞分裂素、应激相关激素以及激素相关基因（ 、 、 、 、 、 和 ）的表达。叶片中的激素浓度会随着去驯化而动态变化，促进生长的激素量增加，应激激素量减少。此外，去驯化过程中基因表达的改变，如 和 中的改变，可能作为一种保护机制，在温暖期过后温度再次下降时重新驯化过程中帮助维持或恢复抗冻性。这些发现增进了我们对油菜去驯化过程中激素和分子反应的理解。
