Ko Min Sung, Eom Da Bin, Lee Chung Hyeon, Park Tae-Eun, Lee Sang Jae, Kim Cheol Hyun, Moon Hui Won, Lee Seo An, Hwang Kwang Woo, Park So-Young
Laboratory of Pharmacognosy, College of Pharmacy, Dankook University, Cheonan 31116, Republic of Korea.
Laboratory of Dairy Science and Technology, College of Bio-Convergence, Dankook University, Cheonan 31116, Republic of Korea.
Molecules. 2025 Jul 25;30(15):3124. doi: 10.3390/molecules30153124.
(commonly known as Cheon-nyun-cho) has traditionally been used for its antioxidant, laxative, and immune-boosting properties, but its potential prebiotic activity remains largely unexplored. In this study, we evaluated the prebiotic potential of leaf and fruit extracts by assessing their effects on the growth of four spp. strains. Among them, KCTC 12576 exhibited the most pronounced response to the extracts and was therefore selected for further investigation. Comparative analysis demonstrated that ethanol extracts were more effective than water extracts, and leaf extracts outperformed fruit extracts in enhancing bacterial viability. Notably, the ethanol extract of leaves showed the strongest stimulatory effect on KCTC 12576 growth. Based on extraction optimization studies, 60% ethanol was identified as the most effective solvent for obtaining bioactive compounds. HPLC analysis revealed the presence of isorhamnetin 3-O-β-D-(6-O-α-L-rhamnosyl)glucoside () as a major flavonol glycoside in the extract. A robust and validated HPLC method was established for quantification of this compound (0.33 mg/g in the 60% ethanol extract of the leaves), supporting the standardization of the extract. These findings suggest that leaf extract, particularly the 60% ethanol extract, may serve as a promising natural prebiotic ingredient for use in functional foods or synbiotic formulations.
（通常被称为天云草）传统上因其抗氧化、通便和增强免疫力的特性而被使用，但其潜在的益生元活性在很大程度上仍未被探索。在本研究中，我们通过评估其对四种菌株生长的影响来评价叶和果实提取物的益生元潜力。其中，KCTC 12576对提取物表现出最显著的反应，因此被选作进一步研究。比较分析表明，乙醇提取物比水提取物更有效，并且叶提取物在提高细菌活力方面优于果实提取物。值得注意的是，叶的乙醇提取物对KCTC 12576的生长显示出最强的刺激作用。基于提取优化研究，60%乙醇被确定为获得生物活性化合物的最有效溶剂。高效液相色谱分析显示提取物中存在异鼠李素3 - O - β - D -（6 - O - α - L - 鼠李糖基）葡萄糖苷（）作为主要的黄酮醇糖苷。建立了一种可靠且经过验证的高效液相色谱方法用于定量该化合物（叶的60%乙醇提取物中为0.33 mg/g），支持提取物的标准化。这些发现表明，叶提取物，特别是60%乙醇提取物，可能作为一种有前景的天然益生元成分用于功能性食品或合生元配方。
