Su Xian-Ni, Wang Yu-Yang, Khan Muhammed Fahad, Zhu Li-Na, Chen Zhong-Liang, Wang Zhuo, Song Bing-Bing, Zhao Qiao-Li, Zhong Sai-Yi, Li Rui
Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524008, China.
Shenzhen Research Institute, Guangdong Ocean University, Shenzhen 518108, China.
Foods. 2025 Jul 26;14(15):2629. doi: 10.3390/foods14152629.
During food processing and storage, traditional protein-based delivery systems encounter significant challenges in maintaining the structural and functional integrity of bioactive compounds, primarily due to their temporal instability. In this study, a nanocomposite hydrogel was prepared through the co-assembly of a self-assembling peptide, 9-Fluorenylmethoxycarbonyl-phenylalanine-arginine-glycine-aspartic acid-phenylalanine (Fmoc-FRGDF), and hyaluronic acid (HA). The stability of this hydrogel as a quercetin (Que) delivery carrier was systematically investigated. Furthermore, the impact of Que co-assembly on the microstructural evolution and physicochemical properties of the hydrogel was characterized. Concurrently, the encapsulation efficiency (EE%) and controlled release kinetics of Que were quantitatively evaluated. The findings indicated that HA significantly reduced the storage modulus (G') from 256.5 Pa for Fmoc-FRGDF to 21.1 Pa with the addition of 0.1 mg/mL HA. Despite this reduction, HA effectively slowed degradation rates; specifically, residue rates of 5.5% were observed for Fmoc-FRGDF alone compared to 14.1% with 0.5 mg/mL HA present. Notably, Que enhanced G' within the ternary complex, increasing it from 256.5 Pa in Fmoc-FRGDF to an impressive 7527.0 Pa in the Que/HA/Fmoc-FRGDF hydrogel containing 0.1 mg/mL HA. The interactions among Que, HA, and Fmoc-FRGDF involved hydrogen bonding, electrostatic forces, and hydrophobic interactions; furthermore, the co-assembly process strengthened the β-sheet structure while significantly promoting supramolecular ordering. Interestingly, the release profile of Que adhered to the Korsmeyer-Peppas pharmacokinetic equations. Overall, this study examines the impact of polyphenol on the rheological properties, microstructural features, secondary structure conformation, and supramolecular ordering within peptide-polysaccharide-polyphenol ternary complexes, and the Fmoc-FRGDF/HA hydrogel system demonstrates a superior performance as a delivery vehicle for maintaining quercetin's bioactivity, thereby establishing a multifunctional platform for bioactive agent encapsulation and controlled release.
在食品加工和储存过程中，传统的基于蛋白质的递送系统在维持生物活性化合物的结构和功能完整性方面面临重大挑战，主要是由于其时间上的不稳定性。在本研究中，通过自组装肽9-芴甲氧羰基-苯丙氨酸-精氨酸-甘氨酸-天冬氨酸-苯丙氨酸（Fmoc-FRGDF）与透明质酸（HA）的共组装制备了一种纳米复合水凝胶。系统研究了这种水凝胶作为槲皮素（Que）递送载体的稳定性。此外，还表征了Que共组装对水凝胶微观结构演变和物理化学性质的影响。同时，定量评估了Que的包封效率（EE%）和控释动力学。研究结果表明，添加0.1 mg/mL HA后，HA显著降低了储存模量（G'），从Fmoc-FRGDF的256.5 Pa降至21.1 Pa。尽管有这种降低，HA有效地减缓了降解速率；具体而言，单独的Fmoc-FRGDF的残留率为5.5%，而存在0.5 mg/mL HA时为14.1%。值得注意的是，Que提高了三元复合物中的G'，从Fmoc-FRGDF中的256.5 Pa增加到含有0.1 mg/mL HA的Que/HA/Fmoc-FRGDF水凝胶中的7527.0 Pa。Que、HA和Fmoc-FRGDF之间的相互作用涉及氢键、静电力和疏水相互作用；此外，共组装过程加强了β-折叠结构，同时显著促进了超分子有序化。有趣的是，Que的释放曲线符合Korsmeyer-Peppas药代动力学方程。总体而言，本研究考察了多酚对肽-多糖-多酚三元复合物中流变学性质、微观结构特征、二级结构构象和超分子有序化的影响，并且Fmoc-FRGDF/HA水凝胶系统作为维持槲皮素生物活性的递送载体表现出优异性能，从而建立了一个用于生物活性剂封装和控释的多功能平台。
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