Wei Yunshan, Zhou Huange, Kang Jingxuan, Wu Yongmei, Feng Kun
College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China.
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China.
Foods. 2025 Jul 26;14(15):2628. doi: 10.3390/foods14152628.
Increased consumer awareness on food safety has spurred the development of detection techniques for pesticide residues. In this study, a rapid detection card on the basis of enzyme action was developed for the visual detection of pesticides, in which the thermally crosslinked and surface-decorated polyvinyl alcohol/citric acid nanofiber mat (PCNM) was employed as a novel immobilization matrix for acetylcholinesterase (AChE). The PCNM, crosslinked at 130 °C for 50 min, exhibited appropriate microstructure and water stability, making it suitable for AChE immobilization. The activation of carboxyl groups by surface decoration resulted in a 2.5-fold increase in enzyme loading capacity. Through parameter optimization, the detection limits for phoxim and methomyl were determined to be 0.007 mg/L and 0.10 mg/L, respectively. The detection card exhibited superior sensitivity and a reduced detection time (11 min) when compared to a commercially available pesticide detection card. Furthermore, the detection results of pesticide residues in fruit and vegetable samples confirmed its feasibility and superiority over commercial alternatives, suggesting its great potential for practical application in the on-site detection of pesticide residues.
消费者对食品安全意识的提高推动了农药残留检测技术的发展。在本研究中，开发了一种基于酶作用的快速检测卡用于农药的可视化检测，其中热交联和表面修饰的聚乙烯醇/柠檬酸纳米纤维毡（PCNM）被用作乙酰胆碱酯酶（AChE）的新型固定化基质。在130℃下交联50分钟的PCNM表现出合适的微观结构和水稳定性，使其适合于AChE的固定化。表面修饰使羧基活化，酶负载量增加了2.5倍。通过参数优化，确定了辛硫磷和灭多威的检测限分别为0.007mg/L和0.10mg/L。与市售农药检测卡相比，该检测卡具有更高的灵敏度和更短的检测时间（11分钟）。此外，水果和蔬菜样品中农药残留的检测结果证实了其相对于商业替代品的可行性和优越性，表明其在农药残留现场检测中的实际应用具有巨大潜力。
