Atiroğlu Vesen, Atiroğlu Atheer, Atiroğlu Ahmed, Al-Hajri Ali Sultan, Özacar Mahmut
Sakarya University, Biomedical, Magnetic and Semiconductor Materials Application and Research Center (BIMAS-RC), 54050, Sakarya, Turkey; Sakarya University, Biomaterials, Energy, Photocatalysis, EnzymeTechnology, Nan &Advanced Materials, Additive Manufacturing, Environmental Applications, and Sustainability Research & Development Group (BIOENAMS R & D Group), 54050, Sakarya, Turkey.
Sakarya University, Biomedical, Magnetic and Semiconductor Materials Application and Research Center (BIMAS-RC), 54050, Sakarya, Turkey; Sakarya University, Biomaterials, Energy, Photocatalysis, EnzymeTechnology, Nan &Advanced Materials, Additive Manufacturing, Environmental Applications, and Sustainability Research & Development Group (BIOENAMS R & D Group), 54050, Sakarya, Turkey.
Food Chem. 2024 Aug 1;448:138978. doi: 10.1016/j.foodchem.2024.138978. Epub 2024 Mar 12.
In the current years of heightened focus on green chemistry and sustainable materials, this study delves into the untapped potential of hyaluronic acid (HA), chitin, and chitosan-prominent polysaccharides for groundbreaking applications. The primary aim is to effectively immobilize catalase enzymes onto matrices composed of chitosan, chitin, HA/chitin, and HA/chitosan. The rigorous investigation covers a spectrum of structural enhancements encompassing pH and temperature stability, thermal resilience, half-life extension, storage durability, reusability, and comprehensive FTIR analyses of the catalase immobilization. Notably, catalase activity demonstrated remarkable resilience on HA/chitin and HA/chitosan matrices, maintaining 73.80% and 79.55% efficacy even after 25 cycles. The introduction of covalent cross-linking between catalase and HA/chitin or HA/chitosan, facilitated by a cross-linking agent, significantly amplified stability and recycling efficiency. Consequently, the immobilized catalase showcases substantial promise across a spectrum of industrial applications, spanning from food and detergent production to bioremediation and diverse commercial processes. This underscores its pivotal role as a versatile and invaluable innovation in the realm of sustainable technologies.
在当前对绿色化学和可持续材料高度关注的几年里，本研究深入探讨了透明质酸（HA）、几丁质和壳聚糖等重要多糖尚未开发的潜力，以实现突破性应用。主要目的是将过氧化氢酶有效地固定在由壳聚糖、几丁质、HA/几丁质和HA/壳聚糖组成的基质上。严格的研究涵盖了一系列结构增强方面，包括pH值和温度稳定性、热弹性、半衰期延长、储存耐久性、可重复使用性以及对过氧化氢酶固定化的全面傅里叶变换红外光谱（FTIR）分析。值得注意的是，过氧化氢酶活性在HA/几丁质和HA/壳聚糖基质上表现出显著的弹性，即使在25个循环后仍保持73.80%和79.55%的功效。通过交联剂促进过氧化氢酶与HA/几丁质或HA/壳聚糖之间的共价交联，显著提高了稳定性和回收效率。因此，固定化过氧化氢酶在从食品和洗涤剂生产到生物修复以及各种商业过程等一系列工业应用中展现出巨大的潜力。这突出了其作为可持续技术领域中一种多功能且极具价值的创新的关键作用。
