Jurášková Dominika, Ribeiro Susana C, Silva Célia C G
Institute of Agricultural and Environmental Research and Technology (IITAA), University of the Azores, 9700-042 Angra do Heroísmo, Portugal.
Foods. 2025 Jul 27;14(15):2635. doi: 10.3390/foods14152635.
The exopolysaccharide (EPS) produced by SJC113 is a glucan with α-1,6 and α-3,6 branched glycosidic linkages that may promote human health. The aim of this study was to investigate in vitro the antioxidant, cholesterol-binding, and prebiotic activities of this EPS and its effect on the gut microbiota. The EPS exhibited moderate antioxidant activity, showing free radical scavenging activity (10.94 ± 1.33%) and hydroxyl scavenging activity (6.29 ± 1.59%) at 1 mg/mL. Notably, it showed high cholesterol-binding activity, lowering cholesterol levels by 40% at 1 mg/mL EPS. SJC113 showed strong adhesion to mucin, and its EPS enhanced the adhesion of the probiotic GG. The application of this EPS stimulated the growth of several lactic acid bacteria (LAB) strains in vitro, indicating its potential as a prebiotic. In addition, the use of a human gastrointestinal simulator inoculated with fecal microbiota showed that the EPS favored the growth of spp. and lactobacilli while reducing . These results emphasize the multifunctional nature of the EPS produced by SJC113 with antioxidant, cholesterol-lowering, and prebiotic properties. Further research is required to investigate the specific mechanisms of action and health benefits in vivo.
SJC113产生的胞外多糖（EPS）是一种具有α-1,6和α-3,6分支糖苷键的葡聚糖，可能促进人类健康。本研究的目的是在体外研究这种EPS的抗氧化、胆固醇结合和益生元活性及其对肠道微生物群的影响。EPS表现出适度的抗氧化活性，在1 mg/mL时显示出自由基清除活性（10.94±1.33%）和羟基清除活性（6.29±1.59%）。值得注意的是，它显示出高胆固醇结合活性，在1 mg/mL EPS时可使胆固醇水平降低40%。SJC113对粘蛋白表现出强烈的粘附性，其EPS增强了益生菌GG的粘附性。这种EPS的应用在体外刺激了几种乳酸菌（LAB）菌株的生长，表明其作为益生元的潜力。此外，使用接种了粪便微生物群的人体胃肠道模拟器表明，EPS有利于spp.和乳酸杆菌的生长，同时减少了。这些结果强调了SJC113产生的EPS具有抗氧化、降胆固醇和益生元特性的多功能性质。需要进一步研究以调查其在体内的具体作用机制和健康益处。
