Tintoré Maria, Cuñé Jordi, Vu Lam Dai, Poppe Jonas, Van den Abbeele Pieter, Baudot Aurélien, de Lecea Carlos
AB Biotek Human Nutrition and Health, Peterborough PE7 8QJ, UK.
Cryptobiotix SA, Technologiepark-Zwijnaarde 82, 9052 Ghent, Belgium.
Biology (Basel). 2024 Jan 18;13(1):51. doi: 10.3390/biology13010051.
Long-chain dextrans are α-glucans that can be produced by lactic acid bacteria. NextDext, a specific long-chain dextran with a high degree of polymerisation, produced using , was recently shown to exert prebiotic potential in vitro. In this study, the ex vivo SIFR technology, recently validated to provide predictive insights into gut microbiome modulation down to the species level, was used to investigate the effects of this long-chain dextran on the gut microbiota of six human adults that altogether covered different enterotypes. A novel community modulation score (CMS) was introduced based on the strength of quantitative 16S rRNA gene sequencing and the highly controlled ex vivo conditions. This CMS overcomes the limitations of traditional α-diversity indices and its application in the current study revealed that dextran is a potent booster of microbial diversity compared to the reference prebiotic inulin (IN). Long-chain dextran not only exerted bifidogenic effects but also consistently promoted spp., and butyrate-producing species like and . Further, long-chain dextran treatment resulted in lower gas production compared to IN, suggesting that long-chain dextran could be better tolerated. The additional increase in for dextran compared to IN is likely related to the higher propionate:acetate ratio, attributing potential to long-chain dextran for improving metabolic health and weight management. Moreover, the stimulation of butyrate by dextran suggests its potential for improving gut barrier function and inflammation. Overall, this study provides a novel tool for assessing gut microbial diversity ex vivo and positions long-chain dextran as a substrate that has unique microbial diversity enhancing properties.
长链葡聚糖是可由乳酸菌产生的α-葡聚糖。NextDext是一种具有高度聚合度的特定长链葡聚糖，最近研究表明，使用[具体方法]生产的这种长链葡聚糖在体外具有益生元潜力。在本研究中，最近经过验证可在物种水平上对肠道微生物群调节提供预测性见解的离体SIFR技术，被用于研究这种长链葡聚糖对六名成年人类肠道微生物群的影响，这六名成年人涵盖了不同的肠型。基于定量16S rRNA基因测序的强度和高度可控的离体条件，引入了一种新的群落调节评分（CMS）。该CMS克服了传统α-多样性指数的局限性，其在当前研究中的应用表明，与参考益生元菊粉（IN）相比，葡聚糖是微生物多样性的有力促进剂。长链葡聚糖不仅发挥了双歧杆菌生成作用，还持续促进了[具体菌种]、[具体菌种]以及如[具体菌种]和[具体菌种]等产丁酸盐的菌种的生长。此外，与IN相比，长链葡聚糖处理导致气体产生量更低，这表明长链葡聚糖可能具有更好的耐受性。与IN相比，葡聚糖中[具体物质]的额外增加可能与较高的丙酸：乙酸比例有关，这赋予了长链葡聚糖改善代谢健康和体重管理的潜力。此外，葡聚糖对丁酸盐的刺激表明其具有改善肠道屏障功能和炎症的潜力。总体而言，本研究提供了一种用于离体评估肠道微生物多样性的新工具，并将长链葡聚糖定位为具有独特微生物多样性增强特性的底物。
