Wacogne Bruno, Belinger Podevin Marine, Vaccari Naïs, Koubevi Claudia, Codjiová Céline, Gutierrez Emilie, Davoine Lucie, Robert-Nicoud Marjorie, Rouleau Alain, Frelet-Barrand Annie
Institut FEMTO-ST, Université de Franche-Comté, CNRS, F-25000 Besançon, France.
Centre d'Investigation Clinique, Centre Hospitalier Universitaire de Besançon, INSERM CIC 1431, 25030 Besançon, France.
Sensors (Basel). 2024 Dec 21;24(24):8160. doi: 10.3390/s24248160.
Optical density measurement has been used for decades to determine the microorganism concentration and more rarely for mammalian cells. Although this measurement can be carried out at any wavelength, studies report a limited number of measurement wavelengths, mainly around 600 nm, and no consensus seems to be emerging to propose an objective method for determining the optimum measurement wavelength for each microorganism. In this article, we propose a method for analyzing the absorbance spectra of ESKAPEE bacteria and determining the optimum measurement wavelength for each of them. The method is based on the analysis of the signal-to-noise ratio of the relationships between concentrations and optical densities when the measurement wavelength varies over the entire spectral range of the absorbance spectra measured for each bacterium. These optimum wavelengths range from 612 nm for to 705 nm for . The method can be directly applied to any bacteria, any culture method, and also to any biochemical substance with an absorbance spectrum without any particular feature such as an identified maximum.
几十年来，光密度测量一直用于确定微生物浓度，而用于哺乳动物细胞的情况则较为少见。尽管这种测量可以在任何波长下进行，但研究报告的测量波长数量有限，主要在600纳米左右，而且似乎没有出现一种共识来提出一种客观方法，以确定每种微生物的最佳测量波长。在本文中，我们提出了一种分析ESKAPEE细菌吸光光谱并确定每种细菌最佳测量波长的方法。该方法基于分析当测量波长在为每种细菌测量的吸光光谱的整个光谱范围内变化时，浓度与光密度之间关系的信噪比。这些最佳波长范围从[具体细菌1]的612纳米到[具体细菌2]的705纳米。该方法可直接应用于任何细菌、任何培养方法，也可应用于任何具有吸光光谱且无任何特定特征（如已确定的最大值）的生化物质。
