Ghazalová Mona, Modlitbová Pavlína, Samek Ota, Rebrošová Katarína, Šiler Martin, Ježek Jan, Pilát Zdeněk
Institute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech Republic.
Department of Microbiology, Faculty of Medicine of Masaryk University and St. Anne's, University Hospital, Pekařská 53, 656 91 Brno, Czech Republic.
Sensors (Basel). 2025 Jul 26;25(15):4629. doi: 10.3390/s25154629.
This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor-demineralized water-inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument.
这项初步研究调查了五种选定细菌对生理应激的代谢反应。表面增强拉曼光谱用于分析与腺嘌呤释放相关的光谱变化，腺嘌呤是一种指示应激条件的关键代谢物。实验室合成的球形银和金纳米颗粒在较长时间内保持稳定，被用作增强表面。在标准条件下以及在选定的应激源——去离子水诱导的渗透应激存在的情况下对细菌培养物进行分析。结果表明，腺嘌呤信号源自释放到周围环境中的代谢物，而非直接来自细菌细胞壁。该研究证实了这些具有成本效益且易于合成的稳定纳米颗粒适用于使用市售拉曼仪器对细菌代谢物进行定性检测。
