Qu Quanying, Li Qi, Xiao Xiao, Zhu Kongli, Gao Wenjie, Wang Qinzhi, Wu Wei
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.
Advanced Agri-Tech Institute, Qingdao Agricultural University, Qingdao, 266109, China.
Mikrochim Acta. 2025 Aug 25;192(9):620. doi: 10.1007/s00604-025-07468-4.
A multifunctional surface-enhanced Raman scattering (SERS) sensor was initially developed by integrating MXene with Au nanoparticles (MXene/Au) and plasmonic metal composite (Au@Ag NFs) as the substrate, combined with aptamers (apt) and signal molecules (4-MBA) for sensitively recognizing S. aureus in milk products. Firstly, MXene possesses the characteristic of a large specific surface area. On the one hand, it provides a higher number of attachment sites for the plasmonic metal composite, enabling the metal nanoparticles to be uniformly distributed on its surface. On the other hand, it exhibits a pre-enrichment effect for the target bacteria, remarkably enhancing the sensitivity of the SERS sensor. Secondly, Au@Ag NFs/4-MBA coupled with MXene/Au produced a synergistic electromagnetic (EM) enhancement effect, which greatly enhanced the SERS intensity and amplified the Raman signal. Furthermore, the thiol-modified aptamer (SH-apt) dramatically enhances the specificity of the SERS sensor system. Based on the sandwich-structured MXene/Au-apt-Au@Ag NFs/4-MBA, S. aureus can be detected with high sensitivity, achieving a low limit of detection of 1.009 CFU/mL and a broad linear detection range from 10 to 10 CFU/mL. Additionally, S. aureus in milk products can be detected with satisfactory recoveries of 86.38-106.65%, which indicates that it has good practical applicability. This method provides an innovative SERS strategy for rapidly monitoring S. aureus in milk products, and exhibits excellent practical applicability as well as potential application value in food safety.
一种多功能表面增强拉曼散射（SERS）传感器最初是通过将MXene与金纳米颗粒（MXene/Au）和等离子体金属复合材料（Au@Ag NFs）集成作为基底，并结合适配体（apt）和信号分子（4-MBA）来灵敏识别乳制品中的金黄色葡萄球菌而开发的。首先，MXene具有大比表面积的特性。一方面，它为等离子体金属复合材料提供了更多的附着位点，使金属纳米颗粒能够均匀分布在其表面。另一方面，它对目标细菌表现出预富集作用，显著提高了SERS传感器的灵敏度。其次，Au@Ag NFs/4-MBA与MXene/Au产生协同电磁（EM）增强效应，极大地增强了SERS强度并放大了拉曼信号。此外，硫醇修饰的适配体（SH-apt）显著提高了SERS传感器系统的特异性。基于三明治结构的MXene/Au-apt-Au@Ag NFs/4-MBA，能够高灵敏度地检测金黄色葡萄球菌，实现了1.009 CFU/mL的低检测限和10至10 CFU/mL的宽线性检测范围。此外，乳制品中的金黄色葡萄球菌检测回收率为86.38 - 106.65%，令人满意，这表明它具有良好的实际适用性。该方法为快速监测乳制品中的金黄色葡萄球菌提供了一种创新的SERS策略，在食品安全方面具有出色的实际适用性以及潜在的应用价值。
