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一种评估药物对鱼类[具体阶段未给出]阶段疗效的新方法。

A novel method for evaluating the efficacy of pharmaceuticals against the stage of in fish.

作者信息

Hu Guang-Ran, Zeng Qing-Wen, Huang Ke, Zou Hong, Li Wen-Xiang, Wu Shan-Gong, Wang Gui-Tang, Li Ming

机构信息

State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology (CAS), and Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.

Guangzhou Customs District Technology Center, Guangzhou, 510623, China.

出版信息

MethodsX. 2025 Jul 2;15:103480. doi: 10.1016/j.mex.2025.103480. eCollection 2025 Dec.

DOI:10.1016/j.mex.2025.103480
PMID:40808758
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12343348/
Abstract

This study presents a description of a novel apparatus for evaluating the efficacy of pharmaceuticals against the stage of the fish parasite . The apparatus comprises a transparent acrylic rectangular cuboid, a metal wire mesh, and a gridded black acrylic bottom plate. Fallen trophonts are caught on the bottom plate and are separated from the fish by the mesh. The efficacy of a pharmaceutical can be assessed by adding it to the aquarium and subsequently counting the number of live tomonts and differntiating rate on the bottom plate. This device facilitates observation and enhances the precision and accuracy of pharmaceutical evaluation experiments.

摘要

本研究介绍了一种用于评估药物对鱼类寄生虫阶段疗效的新型装置。该装置包括一个透明的丙烯酸矩形长方体、一个金属丝网和一个带网格的黑色丙烯酸底板。脱落的滋养体被捕集在底板上,并通过网与鱼分离。通过将药物添加到水族箱中,随后计算底板上活包囊体的数量和分化率,可以评估药物的疗效。该装置便于观察,并提高了药物评估实验的精度和准确性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/31d19ee0ac75/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/5c77ca3a9ade/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/c5318269bd1f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/543bef024197/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/ca736bdea4f3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/31d19ee0ac75/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/5c77ca3a9ade/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/c5318269bd1f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/543bef024197/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/ca736bdea4f3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e8/12343348/31d19ee0ac75/gr4.jpg

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本文引用的文献

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Catechol compounds as dual-targeting agents for fish protection against Ichthyophthirius multifiliis infections.儿茶素类化合物作为鱼类防治多子小瓜虫感染的双重靶向药物。
Fish Shellfish Immunol. 2024 Aug;151:109717. doi: 10.1016/j.fsi.2024.109717. Epub 2024 Jun 22.
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Predation of Cyclopoid Copepods on the Theronts of : Shedding Light on Biocontrol of White Spot Disease.挠足类对白斑病生物防治的研究:剑水蚤对白斑病游动孢子的捕食作用
Pathogens. 2023 Jun 22;12(7):860. doi: 10.3390/pathogens12070860.
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Molecular immune response of channel catfish immunized with live theronts of Ichthyophthirius multifiliis.
用多子小瓜虫活幼虫免疫的斑点叉尾鮰的分子免疫反应
Fish Shellfish Immunol. 2016 Jul;54:86-92. doi: 10.1016/j.fsi.2016.03.166. Epub 2016 Apr 1.
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Minimum effective concentrations of formalin and sodium percarbonate on the free-living stages of an Australian isolate of Ichthyophthirius multifiliis.福尔马林和过碳酸钠对澳大利亚多子小瓜虫分离株自由生活阶段的最低有效浓度
Parasitol Res. 2014 Sep;113(9):3251-8. doi: 10.1007/s00436-014-3987-5. Epub 2014 Jun 20.
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Parasitol Res. 2013 Jun;112(6):2331-40. doi: 10.1007/s00436-013-3397-0. Epub 2013 Apr 5.
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An assessment of the use of drug and non-drug interventions in the treatment of Ichthyophthirius multifiliis Fouquet, 1876, a protozoan parasite of freshwater fish.评估药物和非药物干预措施在治疗多子小瓜虫(Ichthyophthirius multifiliis Fouquet, 1876)中的应用,多子小瓜虫是淡水鱼类的原生动物寄生虫。
Parasitology. 2012 Feb;139(2):149-90. doi: 10.1017/S0031182011001867. Epub 2011 Nov 14.
7
Effect of potassium ferrate(VI) on survival and reproduction of Ichthyophthirius multifiliis tomonts.六价铁酸钾对多子小瓜虫(Ichthyophthirius multifiliis) 多核体存活和繁殖的影响。
Parasitol Res. 2011 Nov;109(5):1423-8. doi: 10.1007/s00436-011-2389-1. Epub 2011 Apr 26.