Josephat Japhet Kimondo, Mpinda Cyprian Beda, Masalu Rose Justus
Department of Molecular Biology and Biotechnology, College of Natural and Applied Science, University of Dar Es salaam, P.O.Box 35179 Dar es Salaam, Tanzania.
Afr Health Sci. 2024 Jun;24(2):124-137. doi: 10.4314/ahs.v24i2.15.
Traditional healers utilize the roots of for managing diabetes mellitus. Therefore, evaluation of toxic properties of this plant is important.
To evaluate acute oral toxicity of root extract on Wistar rats and to screen phytochemical compounds of the EAESZ.
GCMS analysis of the plant extracts were performed by using GCMS-2010 Shimadzu and mass spectra of the compounds found in the extract was matched with the data in the library of National Institute of Standards and Technology (NIST). Acute oral toxicity testing was carried by administering a single Distilled water extract (DWESZ) and EAESZ to four different groups of rats at dosage of 300mg/kg and 2000mg/kg in each extract to the separately group of rats.
The GC-MS analysis of roots extract revealed the presence of 10 major compounds. A higher single dose (2000mg/Kg) of EAESZ and DWESZ extract did not produce any sign of toxicity throughout 14 days of study, in terms of changes in behaviour or mortality in tested rats. No significant (p > 0.05) hematological, liver histological, biochemical changes were noticed between rats treated and control rats.
The results obtained suggest that the plant extract can be classified as non-toxic.
传统治疗师使用[植物名称]的根来治疗糖尿病。因此，评估这种植物的毒性特性很重要。
评估[植物名称]根提取物对Wistar大鼠的急性口服毒性，并筛选该提取物中的植物化学成分。
使用岛津GCMS - 2010对植物提取物进行气相色谱 - 质谱联用（GCMS）分析，并将提取物中发现的化合物的质谱与美国国家标准与技术研究院（NIST）的库中的数据进行匹配。通过以300mg/kg和2000mg/kg的剂量分别向四组不同的大鼠单独给予蒸馏水提取物（DWESZ）和[植物名称]乙醇提取物（EAESZ）来进行急性口服毒性测试。
[植物名称]根提取物的GC - MS分析显示存在10种主要化合物。在为期14天的研究中，较高单剂量（2000mg/Kg）的EAESZ和DWESZ提取物在受试大鼠的行为变化或死亡率方面未产生任何毒性迹象。在受试大鼠和对照大鼠之间未观察到显著（p>0.05）的血液学、肝脏组织学、生化变化。
所得结果表明该植物提取物可归类为无毒。
