Koohkansaadi Ghazal, Mohagheghi Arash, Mobed Ahmad, Charsouei Saeid
Department of Psychology University of New Hampshire Durham New Hampshire USA.
Research Center of Psychiatry and Behavioral Sciences Tabriz University of Medical Sciences Tabriz Iran.
Anal Sci Adv. 2025 Aug 12;6(2):e70035. doi: 10.1002/ansa.70035. eCollection 2025 Dec.
Stroke remains a leading cause of morbidity and mortality worldwide, necessitating the development of rapid and reliable diagnostic tools for early detection and management. This manuscript presents an overview of innovative biosensors designed for the detection of key stroke biomarkers, including N-terminal pro B-type natriuretic peptide (NT-proBNP), C-reactive protein (CRP), D-dimer, cardiac troponins, S100B protein, glial fibrillary acidic protein (GFAP), neurofilament light chain (NfL), matrix metalloproteinases (MMPs), interleukins and fibrinogen. We discuss the principles of operation, sensitivity, specificity and the technological advancements that have enabled the development of these biosensors, including electrochemical, optical and microfluidic platforms. The integration of nanomaterials and advanced signal amplification techniques has significantly enhanced the performance of these biosensors, allowing for the detection of biomarkers at low concentrations in complex biological samples. Furthermore, we explore the clinical implications of these biosensors in the context of stroke diagnosis, prognosis and monitoring, highlighting their potential to facilitate timely therapeutic interventions. By providing a comprehensive discussion on the current state of biosensor technology for stroke biomarker detection, this manuscript aims to underscore the importance of these tools in improving patient outcomes and advancing stroke research. Future directions for biosensor development and the challenges that remain in translating these technologies into clinical practice are also addressed.
中风仍然是全球发病和死亡的主要原因，因此需要开发快速可靠的诊断工具用于早期检测和管理。本手稿概述了为检测关键中风生物标志物而设计的创新型生物传感器，这些生物标志物包括N末端B型利钠肽原（NT-proBNP）、C反应蛋白（CRP）、D-二聚体、心肌肌钙蛋白、S100B蛋白、胶质纤维酸性蛋白（GFAP）、神经丝轻链（NfL）、基质金属蛋白酶（MMPs）、白细胞介素和纤维蛋白原。我们讨论了这些生物传感器的工作原理、灵敏度、特异性以及推动其发展的技术进步，包括电化学、光学和微流控平台。纳米材料和先进信号放大技术的整合显著提高了这些生物传感器的性能，使其能够在复杂生物样品中检测低浓度的生物标志物。此外，我们探讨了这些生物传感器在中风诊断、预后和监测方面的临床意义，强调了它们在促进及时治疗干预方面的潜力。通过全面讨论用于中风生物标志物检测的生物传感器技术的现状，本手稿旨在强调这些工具在改善患者预后和推进中风研究方面的重要性。还讨论了生物传感器开发的未来方向以及将这些技术转化为临床实践中仍然存在的挑战。
