Paredes-Chávez Reyna Perla, Mata-Sotres José Antonio, Parra-Laca Rocío, Ramírez-Torrez José Alberto, Becerril-Cortés Daniel, Dosta María Del Carmen Monroy
Man and His Environment Department, Metropolitan Autonomous University Xochimilco Unit, Calzada del Hueso, 1100 C.P. 04960, Coyoacan, Mexico.
Science and Technology Faculty, Simon Bolivar México, Av., Rio Mixcoac N° 48, Col. Insurgentes Mixcoac, Benito Juarez C.P. 03920, Mexico, Mexico.
Aquac Nutr. 2025 Aug 6;2025:1521301. doi: 10.1155/anu/1521301. eCollection 2025.
The aim of this study was to evaluate the use of larvae meal as a carbon source in a biofloc system (BFT) to optimize the culture of . A trial was conducted for 24 weeks, 500 larvae were reared with wheat bran as substrate, and hydration once a week; at the end, 9000 larvae were obtained, which were processed to make meal. was conditioned for 2 weeks in two 100 L polyethylene tanks in clear water and fed with Tropical shrimp sticks until the beginning of the experiment. A 12-week experiment was performed with three treatments and three replicates each: control (no biofloc), biofloc with molasses (BFT-M), and biofloc with meal (BFT-T) as carbon source, at a 20:1 C/N ratio, adjusted every 15 days based on the feed ratio. The culture parameters of the water during the experimental period varied, but they always remained within the range required for the species. No significant differences were observed in weight gain (WG) among the three treatments ( > 0.05), but the crayfish cultured in the treatments with biofloc obtained the greatest WG and the lowest feed conversion ratio (FCR). In the biofloc, nine phyla of zooplankton were identified, of which 16 genera were present in BFT-M and 13 in BFT-T. The chitinolytic bacteria showed a greater diversity and richness in BFT-T compared to the BFT-M and control. was the dominant species, and the second one. The nutritional composition (lipids, carbohydrates, fiber, ash, and moisture) of as well as biofloc from the BFT-M and BFT-T showed significant differences ( < 0.05), but not in protein levels. This study demonstrated that meal is a viable carbon source in biofloc culture, improving biofloc nutritional quality through increased chitinolytic bacterial diversity.
本研究的目的是评估在生物絮团系统（BFT）中使用幼虫粉作为碳源以优化[具体生物名称未给出]养殖的情况。进行了为期24周的试验，以麦麸为底物饲养500只[具体幼虫名称未给出]幼虫，每周补水一次；最后获得了9000只幼虫，并将其加工制成粉末。[具体生物名称未给出]在两个100升的聚乙烯水箱中在清水中驯化2周，并投喂热带虾棒直至实验开始。进行了一项为期12周的实验，设置三个处理组，每个处理组三个重复：对照组（无生物絮团）、添加糖蜜的生物絮团组（BFT-M）和添加幼虫粉的生物絮团组（BFT-T）作为碳源，碳氮比为20:1，每15天根据投喂比例进行调整。实验期间水体的养殖参数有所变化，但始终保持在该物种所需的范围内。三种处理组之间的体重增加（WG）未观察到显著差异（P＞0.05），但在添加生物絮团的处理组中养殖的小龙虾体重增加最大且饲料转化率（FCR）最低。在生物絮团中，鉴定出了九个浮游动物门类，其中BFT-M中有16个属，BFT-T中有13个属。与BFT-M和对照组相比，BFT-T中的几丁质分解细菌表现出更高的多样性和丰富度。[具体优势种未给出]是优势种，[具体次优势种未给出]是第二优势种。幼虫粉以及BFT-M和BFT-T中的生物絮团的营养成分（脂质、碳水化合物、纤维、灰分和水分）显示出显著差异（P＜0.05），但蛋白质水平无差异。本研究表明，幼虫粉是[具体生物名称未给出]生物絮团养殖中一种可行的碳源，通过增加几丁质分解细菌的多样性提高了生物絮团的营养质量。
