Zhang Luyue, Du Xuyang, Wang Aowei, Xu Yunfei, Wu Tianyi, Liu Ziqi, Shi Gongyao, Wei Fang, Tian Baoming
Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
J Agric Food Chem. 2025 Aug 27;73(34):21681-21696. doi: 10.1021/acs.jafc.5c05670. Epub 2025 Aug 14.
Cold stress is a major abiotic factor limiting plant growth and productivity. MYB transcription factors are important regulators of stress responses. Our previous study identified SlMYB15 as a positive regulator of cold tolerance in tomato. However, their downstream regulatory mechanisms remained unclear. Herein, subcellular localization confirmed that SlMYB15 is a nuclearly localized transcription factor. Transcriptomic analysis revealed that SlMYB15 modulates genes involved in redox processes, hormone signaling, and glutathione metabolism under cold stress. SlMYB15 upregulated , , , and , reducing ROS accumulation by enhancing antioxidant enzyme activities and modulating -dependent HO signaling. Silencing or impaired the cold tolerance in SlMYB15-overexpressing plants. Furthermore, SlMYB15 activated the expression of and by directly binding to the promoter regions. Collectively, these findings suggest that SlMYB15 enhances cold tolerance by regulating key redox-related genes, providing new insights into MYB-mediated stress resistance in tomato.
低温胁迫是限制植物生长和生产力的主要非生物因素。MYB转录因子是应激反应的重要调节因子。我们之前的研究确定SlMYB15是番茄耐寒性的正向调节因子。然而，其下游调控机制仍不清楚。在此，亚细胞定位证实SlMYB15是一种定位于细胞核的转录因子。转录组分析表明，SlMYB15在低温胁迫下调节参与氧化还原过程、激素信号传导和谷胱甘肽代谢的基因。SlMYB15上调了 、 、 和 ，通过增强抗氧化酶活性和调节依赖 的HO信号传导来减少ROS积累。沉默 或 会削弱SlMYB15过表达植物的耐寒性。此外，SlMYB15通过直接结合启动子区域激活 和 的表达。总体而言，这些发现表明SlMYB15通过调节关键的氧化还原相关基因增强耐寒性，为番茄中MYB介导的抗逆性提供了新见解。
