Watson Alice H, Gilligan Lorna C, Taylor Angela E, Arlt Wiebke, Syme Harriet M
Royal Veterinary College, London, UK.
Queen Mary, University of London, London, UK.
J Vet Intern Med. 2025 Sep-Oct;39(5):e70209. doi: 10.1111/jvim.70209.
Multi-steroid profiles are proven to help with the diagnostic approach to adrenal disorders in humans.
Compare liquid chromatography tandem mass spectrometry (LC-MS/MS) and radioimmunoassay (RIA) measurement of aldosterone in feline blood samples. Evaluate multi-steroid profiles in cats with hyperaldosteronism.
Client-owned cats with low/normal aldosterone by RIA (low aldo, n = 15), high aldosterone by RIA (high aldo, n = 6) and overt primary hyperaldosteronism (PHA, n = 6).
Exploratory retrospective case-control study. Feline blood multi-steroid profiles were analyzed with LC-MS/MS and compared with results from a commercially available RIA in widespread clinical use.
Aldosterone measurement by LC-MS/MS and RIA was strongly correlated (r = 0.93, p < 0.001) but showed a bias of 56 (95% CI: 104-7) pmol/L, with RIA giving higher values. Creatinine concentration was not significantly associated with the discrepancy between methods (p = 0.95). Cats with PHA had significantly lower serum concentrations of cortisol and glucocorticoid precursors (17-hydroxyprogesterone, 11-deoxycortisol), testosterone, and significantly higher dihydrotestosterone and 5α-dihydroprogesterone concentrations than the low aldo group. Pregnenolone concentrations were significantly lower in the PHA group than in the high aldo group.
Quantification of aldosterone by either LC-MS/MS or RIA is adequate for PHA diagnosis in the clinical setting, with appropriate method-specific reference intervals. Cats with PHA had different concentrations of some steroids compared with the control groups, but overt steroid excess (other than aldosterone) was not observed.
多种类固醇谱已被证明有助于人类肾上腺疾病的诊断方法。
比较液相色谱串联质谱法（LC-MS/MS）和放射免疫分析法（RIA）对猫血样中醛固酮的测量。评估醛固酮增多症猫的多种类固醇谱。
通过RIA检测醛固酮水平低/正常的客户拥有的猫（低醛固酮组，n = 15）、通过RIA检测醛固酮水平高的猫（高醛固酮组，n = 6）和明显原发性醛固酮增多症（PHA）的猫（n = 6）。
探索性回顾性病例对照研究。用LC-MS/MS分析猫血多种类固醇谱，并与广泛临床应用的市售RIA结果进行比较。
LC-MS/MS和RIA对醛固酮的测量具有强相关性（r = 0.93，p < 0.001），但存在56（95%CI：104 - 7）pmol/L的偏差，RIA给出的值更高。肌酐浓度与方法间差异无显著相关性（p = 0.95）。与低醛固酮组相比，PHA猫的血清皮质醇和糖皮质激素前体（17-羟孕酮、11-脱氧皮质醇）、睾酮浓度显著降低，二氢睾酮和5α-二氢孕酮浓度显著升高。PHA组孕烯醇酮浓度显著低于高醛固酮组。
在临床环境中，通过LC-MS/MS或RIA对醛固酮进行定量分析足以诊断PHA，并具有适当的方法特异性参考区间。与对照组相比，PHA猫的某些类固醇浓度不同，但未观察到明显的类固醇过量（醛固酮除外）。
