班巴拉花生果皮衍生的碳点：明胶/聚乳酸双层膜的增强及其在亚洲海鲈切片包装中作为防腐剂的功能。

Bambara Groundnut Pericarp-Derived Carbon Dots: Reinforcement of Gelatin/PLA Bilayer Films and Their Function as Preservative in Packed Asian Seabass Slices.

作者信息

Murugan Gokulprasanth, Nilsuwan Krisana, Kim Jun Tae, Rhim Jong-Whan, Yin Tao, Zhang Bin, Benjakul Soottawat

机构信息

International Center of Excellence in Seafood Science and Innovation (ICE-SSI), Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, Thailand.

BioNanocomposite Research Center, Department of Food and Nutrition, Kyung Hee University, Seoul, Republic of Korea.

出版信息

J Food Sci. 2025 Aug;90(8):e70469. doi: 10.1111/1750-3841.70469.

DOI:10.1111/1750-3841.70469

PMID:40814738

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12355346/

Abstract

Conversion of agro-waste into carbon dots (CDs) transforms biomass into sustainable nanomaterials, enabling advanced applications in packaging and ensuring food safety. Bambara groundnut pericarp powder (BGPP) was hydrothermally processed at 200°C for varying durations (3-12 h) to synthesize BGP-CDs. BGP-CDs exhibited differences in size (1.34-15.02 nm), shape, and chemical composition, as characterized by transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) analyses. All samples exhibited excellent optical properties, including photoluminescence and UV barrier capability. Notably, BGP-CDs/3 (synthesized for 3 h) at 100 µg/mL demonstrated the highest UV-blocking efficiency against UV-A (35.62%) and UV-B (92.64%) lights. BGP-CDs synthesized for 12 h exhibited remarkable antimicrobial activities against selected species (Escherichia coli, Listeria monocytogenes, Aspergillus flavus, and Aspergillus parasiticus) due to their nanoscale size; however, their reduced size negatively impacted human cell viability. Antioxidant activity was highest in BGP-CDs synthesized for 6 h (BGP-CDs/6), as determined by different assays. Incorporating BGP-CDs/6 (4%, w/w) into gelatin/polylactic acid (PLA) bilayer film enhanced UV and water barrier properties, antioxidant capacity, and antibacterial efficacy compared to the control film. Additionally, Asian seabass slices (AS-S) packaged in pouches made from active bilayer film had the maintained color with negligible pH change. Microbial growth and lipid oxidation in AS-S were retarded as indicated by the lower rise in total viable count (TVC) and psychrophilic bacterial count (PBC) as well as thiobarbituric acid reactive substances (TBARS) values over 12 days of storage at 4°C. Thus, the developed multifunctional packaging film offers an innovative strategy that could impede the quality loss of perishable fish slices.

摘要

将农业废弃物转化为碳点（CDs）可将生物质转化为可持续的纳米材料，使其在包装领域有先进应用并确保食品安全。对 Bambara 花生果皮粉（BGPP）在 200°C 下进行不同时长（3 - 12 小时）的水热加工，以合成 BGP - CDs。通过透射电子显微镜（TEM）和傅里叶变换红外（FTIR）分析表征，BGP - CDs 在尺寸（1.34 - 15.02 纳米）、形状和化学成分上存在差异。所有样品都表现出优异的光学性能，包括光致发光和紫外线阻隔能力。值得注意的是，浓度为 100 µg/mL 的 BGP - CDs/3（合成 3 小时）对紫外线 A（35.62%）和紫外线 B（92.64%）的阻隔效率最高。合成 12 小时的 BGP - CDs 因其纳米级尺寸对选定物种（大肠杆菌、单核细胞增生李斯特菌、黄曲霉和寄生曲霉）表现出显著的抗菌活性；然而，其尺寸减小对人类细胞活力产生了负面影响。通过不同测定方法确定，合成 6 小时的 BGP - CDs（BGP - CDs/6）抗氧化活性最高。与对照膜相比，将 BGP - CDs/6（4%，w/w）掺入明胶/聚乳酸（PLA）双层膜可增强紫外线和水阻隔性能、抗氧化能力以及抗菌效果。此外，用活性双层膜制成的袋子包装的亚洲海鲈鱼片（AS - S）颜色保持不变，pH 变化可忽略不计。在 4°C 下储存 12 天期间，AS - S 中的总活菌数（TVC）、嗜冷菌数（PBC）以及硫代巴比妥酸反应物质（TBARS）值上升较低，表明 AS - S 中的微生物生长和脂质氧化受到抑制。因此，所开发的多功能包装膜提供了一种创新策略，可阻止易腐鱼片的质量损失。