利用屠宰场来源的卵巢进行绵羊体外胚胎生产的方案。
Protocol for in vitro embryo production in sheep using slaughterhouse-derived ovaries.
作者信息
Ebrahimi Mohammadreza, Mara Laura, Chessa Fabrizio, Angioi Antonio G, Berlinguer Fiammetta, Dattena Maria
机构信息
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, Sassari, Italy; Department of Animal Science, Agricultural Research Agency of Sardinia, 07100 Sassari, Italy; School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD Loughborough, UK.
Department of Animal Science, Agricultural Research Agency of Sardinia, 07100 Sassari, Italy.
出版信息
STAR Protoc. 2025 Aug 14;6(3):104027. doi: 10.1016/j.xpro.2025.104027.
Here, we present a protocol for in vitro embryo production (IVEP) in sheep using slaughterhouse-derived ovaries. We describe steps for ovary collection and transportation under controlled conditions. We then detail procedures for oocyte retrieval, selection, and in vitro maturation. Mature oocytes undergo fertilization with swim-up-processed sperm, and the resulting zygotes are cultured in a commercial medium. This approach ensures high reproducibility and developmental success, yielding cleavage and blastocyst rates over 90% and 60%.
在此，我们介绍一种利用屠宰场来源的卵巢进行绵羊体外胚胎生产（IVEP）的方案。我们描述了在可控条件下卵巢采集和运输的步骤。然后详细说明了卵母细胞检索、选择和体外成熟的程序。成熟卵母细胞与经上浮处理的精子受精，所得合子在商业培养基中培养。这种方法确保了高重复性和发育成功率，卵裂率和囊胚率分别超过90%和60%。
Zotero 插件