da Silva Ayla Winnie Ramos, da Silva Lorena Batista, Rambo Douglas Fernando, Biegelmeyer Renata
Medical Materia Research Laboratory, College of Pharmacy, Federal University of Bahia, 147 Barão de Jeremoabo, Salvador, 40170-115, Brazil.
J Pharm Pharmacol. 2025 Aug 16. doi: 10.1093/jpp/rgaf067.
Natural products (NP) play a crucial role in the development of new compounds, due to their complex chemical structure and pharmacological diversity. Neurodegenerative diseases and other disorders in the central nervous system (CNS) have become a significant problem in the world due to the increase in life expectancy of the elderly population. This increases the risk of developing diseases, such as Parkinson's disease, Huntington's disease, Alzheimer's disease. Therefore, this exploratory review aims to show the applications of zebrafish for NP research and how they can be used in CNS's in vivo studies.
The present review covers the literature survey until 2023, including the descriptors for zebrafish, natural product and neurodegenerative diseases. The databases used were PubMed, Scopus, Cochrane and Lilacs.
For the development of new medicines, an efficient animal model is required, and the zebrafish has stood out as a promising model due to its small size, low cost of maintenance, ease of handling, and transparency of embryos, which allows real-time observation of development and pathological processes. They possess conserved neurotransmission systems such as glutamatergic, cholinergic, dopaminergic, serotonergic, histaminergic, GABAergic, and purinergic pathways, making them especially relevant for modelling CNS disorders. From literature survey, flavonoids, alkaloids, and phenolic compounds were the most frequently studied, indicating that its influence the pathophysiological mechanisms associated with neurodegenerative diseases.
This current review offers data for further research work with natural products aiming treatment for CNS disorders.
天然产物因其复杂的化学结构和药理多样性，在新化合物的研发中发挥着关键作用。由于老年人口预期寿命的增加，神经退行性疾病和中枢神经系统（CNS）的其他疾病已成为全球的一个重大问题。这增加了患帕金森病、亨廷顿舞蹈症、阿尔茨海默病等疾病的风险。因此，本探索性综述旨在展示斑马鱼在天然产物研究中的应用，以及它们如何用于中枢神经系统的体内研究。
本综述涵盖截至2023年的文献调查，包括斑马鱼、天然产物和神经退行性疾病的描述符。使用的数据库有PubMed、Scopus、Cochrane和Lilacs。
为了开发新药，需要一种高效的动物模型，而斑马鱼因其体型小、饲养成本低、易于操作以及胚胎透明，能够实时观察发育和病理过程，已成为一种有前景的模型。它们拥有保守的神经传递系统，如谷氨酸能、胆碱能、多巴胺能、5-羟色胺能、组胺能、γ-氨基丁酸能和嘌呤能途径，这使得它们在模拟中枢神经系统疾病方面特别有意义。从文献调查来看，黄酮类、生物碱和酚类化合物是研究最频繁的，表明它们影响与神经退行性疾病相关的病理生理机制。
本综述为进一步开展旨在治疗中枢神经系统疾病的天然产物研究工作提供了数据。
