Afkhami Hamed, Ahmadi Mohammad Hossein, Owlia Parviz, Saderi Horieh
Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.
Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, Iran.
Curr Microbiol. 2025 Aug 18;82(10):459. doi: 10.1007/s00284-025-04420-w.
This study investigates the antibacterial potential of murine bone marrow-derived mesenchymal stem cells (BM-MSCs) encapsulated in collagen-fibrin hydrogel scaffolds for treating Peptostreptococcus anaerobius-infected full-thickness wounds. P. anaerobius, a Gram-positive anaerobic coccus, is a significant contributor to wound infections and exhibits considerable antibiotic resistance. Given the rising prevalence of antibiotic-resistant bacteria, there is an urgent need for effective non-antibiotic treatments. One of the non-antibiotic treatments for drug-resistant bacteria is the use of mesenchymal stem cells (MSCs). BM-MSCs were isolated, characterized using CD44, CD90, and CD105 markers, and encapsulated in collagen-fibrin scaffolds. Wound healing progression was monitored over 20 days, and bacterial load reduction was quantified using log CFU/g measurements. Our findings demonstrate a significant reduction in bacterial load (up to 123.33 log CFU/g) and complete wound closure by Day 20, underscoring the dual role of this approach in mitigating microbial infection and promoting wound healing. While scaffold stability and degradation were not directly measured, the observed therapeutic outcomes suggest that the hydrogel remained functional and biocompatible for a sufficient duration to support cell activity and tissue regeneration. These results highlight the innovative potential of BM-MSCs encapsulated in collagen-fibrin hydrogel scaffolds as an effective antibacterial therapy for P. anaerobius-infected wounds.
本研究调查了包裹在胶原-纤维蛋白水凝胶支架中的小鼠骨髓间充质干细胞（BM-MSCs）对治疗厌氧消化链球菌感染的全层伤口的抗菌潜力。厌氧消化链球菌是一种革兰氏阳性厌氧球菌，是伤口感染的重要致病菌，且表现出相当强的抗生素耐药性。鉴于抗生素耐药菌的日益流行，迫切需要有效的非抗生素治疗方法。针对耐药菌的非抗生素治疗方法之一是使用间充质干细胞（MSCs）。分离出BM-MSCs，使用CD44、CD90和CD105标志物进行表征，并将其包裹在胶原-纤维蛋白支架中。在20天内监测伤口愈合进程，并使用每克菌落形成单位（log CFU/g）测量来量化细菌载量的降低情况。我们的研究结果表明，到第20天时细菌载量显著降低（高达123.33 log CFU/g）且伤口完全愈合，突出了这种方法在减轻微生物感染和促进伤口愈合方面的双重作用。虽然未直接测量支架的稳定性和降解情况，但观察到的治疗结果表明，水凝胶在足够长的时间内保持功能和生物相容性，以支持细胞活性和组织再生。这些结果突出了包裹在胶原-纤维蛋白水凝胶支架中的BM-MSCs作为厌氧消化链球菌感染伤口的有效抗菌疗法的创新潜力。
