Feng Yuxuan, Bao Mingyuan, Sheng Wenyi, Liu Shiqi, Cui Yuchen, Zhou Nianchao, Wu Tiantian, Huang Xiaoyan, Hou Shunyu, Liu Meng
State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University Nanjing 211166, Jiangsu, China.
Department of Thyroid and Breast Surgery, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University Suzhou 215002, Jiangsu, China.
Am J Transl Res. 2025 Jul 25;17(7):5614-5624. doi: 10.62347/CLIG5523. eCollection 2025.
Defective in cullin neddylation 1 domain containing 2 (DCUN1D2) belongs to the DCNLs family and can induce cell growth arrest and is also related to neddylation. While its potential role in spermatogenesis is hypothesized, the functional significance of DCUN1D2 in male germ cells remains undefined.
To investigate this, germ cell and Sertoli cell-specific -knockouts were generated. Sperm parameters were analyzed via computer-assisted sperm analysis (CASA), while histological and immunofluorescence staining were employed to evaluate spermatogenic progression and apoptosis.
Compared with the control group, conditional knockout (cKO) mice exhibited no significant differences in histology, semen quality, sperm apoptosis or fertility tests.
This study indicated that DCUN1D2 has no significant effect on mouse spermatogenesis or male fertility. These findings will help avoid redundant studies and provide new information for the study of human fertility genes.
含cullin泛素化激活酶1结构域蛋白2（DCUN1D2）属于DCNLs家族，可诱导细胞生长停滞，且与泛素化有关。虽然推测其在精子发生过程中具有潜在作用，但DCUN1D2在雄性生殖细胞中的功能意义仍不明确。
为研究此问题，构建了生殖细胞和支持细胞特异性敲除小鼠。通过计算机辅助精子分析（CASA）分析精子参数，同时采用组织学和免疫荧光染色评估精子发生进程和细胞凋亡情况。
与对照组相比，条件性敲除（cKO）小鼠在组织学、精液质量、精子凋亡或生育力测试方面均无显著差异。
本研究表明，DCUN1D2对小鼠精子发生或雄性生育力无显著影响。这些发现将有助于避免冗余研究，并为人类生育基因的研究提供新信息。
