E3 泛素连接酶 FBXO22 对小鼠精子发生和雄性生育力并不重要。
E3 ligase FBXO22 is not significant for spermatogenesis and male fertility in mice.
作者信息
Wu Tiantian, Jin Xin, Huang Chao, Yu Xiangling, Xu Bingya, Gao Wenxin, Qiu Xiya, Bao Mingyuan, Zhao Dan, Feng Guannan, Zheng Bo, Huang Xiaoyan
机构信息
State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University Nanjing 211166, Jiangsu, China.
Department of Obstetrics and Gynecology, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University Suzhou 215002, Jiangsu, China.
出版信息
Am J Transl Res. 2024 May 15;16(5):1834-1844. doi: 10.62347/STDA4237. eCollection 2024.
BACKGROUND
F-box-only protein 22 (FBXO22), an important substrate receptor of the SKP1-Cullin-F-box (SCF) ubiquitin ligases, has been reported to be involved in many biological processes, including tumorigenesis, neurological disorders, cellular senescence, and DNA damage. However, the specific role of FBXO22 during spermatogenesis is poorly understood.
METHODS
We produced conditional knockout (cKO) and global knockout (KO) mice and assessed their sperm masurements using a computer-assisted sperm analysis (CASA) system. Additionally, we conducted histologic staining and immunostaining to examine the impact of loss on spermatogenesis.
RESULTS
Our results revealed that there were no notable differences in semen quality, fertility test results, or histologic findings in -KO and -cKO mice compared to the control group.
CONCLUSIONS
Our study demonstrated that is not significant for spermatogenesis or male fertility in mice. These findings will help researchers avoid redundant efforts and serve as a foundational resource for genetic studies on human fertility.
背景
仅含F盒蛋白22（FBXO22）是SKP1 - Cullin - F盒（SCF）泛素连接酶的重要底物受体，据报道其参与许多生物学过程，包括肿瘤发生、神经紊乱、细胞衰老和DNA损伤。然而，FBXO22在精子发生过程中的具体作用尚不清楚。
方法
我们制备了条件性敲除（cKO）和全身性敲除（KO）小鼠，并使用计算机辅助精子分析（CASA）系统评估它们的精子指标。此外，我们进行了组织学染色和免疫染色，以检查敲除对精子发生的影响。
结果
我们的结果显示，与对照组相比，-KO和-cKO小鼠在精液质量、生育力测试结果或组织学发现方面没有显著差异。
结论
我们的研究表明， 对小鼠的精子发生或雄性生育力并不重要。这些发现将有助于研究人员避免多余的工作，并为人类生育力的基因研究提供基础资源。
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