Tanaka H, Kobayashi N, Maekawa T
Thromb Res. 1985 Nov 1;40(3):291-306. doi: 10.1016/0049-3848(85)90265-8.
Effect of thrombin and endotoxin on the metabolism of I-125-labelled canine AT III was studied in mongrel dogs. Under control condition, mean total amount of intravascular AT III with standard deviation was 23.4 +/- 2.4 mg/kg, plasma half life of i.v. injected I-125-AT III was 1.7 +/- 0.2 days, and the fractional catabolic flux (j3x) was 16.3 +/- 1.6 mg/kg/day. The total amount of intra- and extra-vascular AT III was 36.0 +/- 0.34 mg/kg. Neither a 3 hour infusion of a small dose (30 units/kg/hr) of thrombin nor i.v. injection of a large amount of thrombin (5,000-15,000 units/day) with heparin significantly affected AT III metabolism except for a transient decrease in AT III concentration in the latter case, although decrease in plasma fibrinogen concentration and platelet count was observed in both cases. Two injections with 200 micrograms/kg of endotoxin resulted in an evident acceleration of AT III metabolism with significant decrease in the plasma AT III, fibrinogen concentrations and platelet count. More marked changes in AT III metabolism were induced by a single infusion with 1 mg/kg of endotoxin. Changes in hemostatic system coincided with those observed in DIC.
在杂种犬中研究了凝血酶和内毒素对I - 125标记的犬抗凝血酶III（AT III）代谢的影响。在对照条件下，血管内AT III的平均总量及其标准差为23.4±2.4mg/kg，静脉注射I - 125 - AT III的血浆半衰期为1.7±0.2天，分解代谢率（j3x）为16.3±1.6mg/kg/天。血管内和血管外AT III的总量为36.0±0.34mg/kg。小剂量（30单位/kg/小时）凝血酶3小时输注或与肝素一起静脉注射大量凝血酶（5000 - 15000单位/天）均未显著影响AT III代谢，只是在后一种情况下AT III浓度有短暂下降，尽管在两种情况下均观察到血浆纤维蛋白原浓度和血小板计数下降。两次注射200μg/kg内毒素导致AT III代谢明显加速，血浆AT III、纤维蛋白原浓度和血小板计数显著降低。单次输注1mg/kg内毒素诱导了更明显的AT III代谢变化。止血系统的变化与在弥散性血管内凝血（DIC）中观察到的变化一致。
