Ružanović Ana, Sarić-Matutinović Marija, Milinković Neda, Jovičić Snežana, Dimić Andreja, Matejević David, Kostić Ognjen, Gaković Branko, Končar Igor, Ignjatović Svetlana
University Clinical Center of Serbia, Clinic for Vascular and Endovascular Surgery, Belgrade.
University of Belgrade, Faculty of Pharmacy, Belgrade.
J Med Biochem. 2025 Jun 13;44(3):553-560. doi: 10.5937/jomb0-51391.
Symptom risk assessment in carotid artery stenosis (CAS) could be improved by parameters that reflect additional risk aspects such as chronic inflammation rate, and atherosclerotic activity on a systemic level. In light of that, we investigated the association of serum matrix metalloproteinases-2,7,9 (MMP-2,7,9), vascular cell adhesion molecule-1 (VCAM-1) and selectins-P and E with symptomatic status, stenosis degree and plaque morphology in CAS patients in order to select parameters that associate to important clinical determinants of the symptom development risk.
The study included 119 CAS patients and 46 healthy subjects. Carotid arteries were examined by color flow Doppler and B-mode Duplex ultrasound. Serum parameters were assessed using commercially available enzyme-linked immunosorbent assays (ELISA). Difference was tested by Mann-Whitney U, Kruskal-Wallis and Chi-square tests, and Spearman's correlation was tested.
MMP-7 and selectin-P levels were higher in CAS than in controls (p<0.001). Positive correlation with stenosis degree was found for MMP-7 (r=0.155, p=0.007), VCAM-1 (r=0.127, p=0.029) and selectin-P (r=0.269, p<0.001). MMP-7 and selectin-P were higher in subjects with Grey-Weale 2, comparing to subjects with Grey-Weale 3 plaques (p=0.036, p=0.009). Selectin-P was lower in the presence of Grey-Weale 4 than in Grey-Weale 2 (p=0.045).
Concurrent association of MMP-7 and selectin-P with both stenosis degree and carotid plaque morphology shows the joint influence of these important determinants of symptom risk that is reflected in serum parameters. This indicates that they can supply additional information outside ultrasound CAS assessment only, and their integration in a future multiscale approach for CAS risk prediction could be beneficial.
反映慢性炎症率和全身水平动脉粥样硬化活动等额外风险因素的参数，可改善颈动脉狭窄（CAS）的症状风险评估。鉴于此，我们研究了血清基质金属蛋白酶-2、7、9（MMP-2、7、9）、血管细胞粘附分子-1（VCAM-1）以及选择素-P和E与CAS患者症状状态、狭窄程度和斑块形态之间的关联，以便选择与症状发生风险的重要临床决定因素相关的参数。
该研究纳入了119例CAS患者和46名健康受试者。通过彩色多普勒血流显像和B型双功超声检查颈动脉。使用市售酶联免疫吸附测定（ELISA）评估血清参数。采用曼-惠特尼U检验、克鲁斯卡尔-沃利斯检验和卡方检验进行差异检验，并进行斯皮尔曼相关性检验。
CAS患者的MMP-7和选择素-P水平高于对照组（p<0.001）。发现MMP-7（r=0.155，p=0.007）、VCAM-1（r=0.127，p=0.029）和选择素-P（r=0.269，p<0.001）与狭窄程度呈正相关。与格雷-韦尔3级斑块的受试者相比，格雷-韦尔2级受试者的MMP-7和选择素-P更高（p=0.036，p=0.009）。存在格雷-韦尔4级时的选择素-P低于格雷-韦尔2级（p=0.045）。
MMP-7和选择素-P与狭窄程度和颈动脉斑块形态同时存在关联，表明这些症状风险的重要决定因素的共同影响可在血清参数中体现。这表明它们不仅可以在超声CAS评估之外提供额外信息，并且将它们整合到未来的CAS风险预测多尺度方法中可能会有益。
