Guan Jian, Wu Shenglin, Liang Renyi
Graduate School, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, China.
Department of Plastic Surgery, Lishui People's Hospital, Lishui, Zhejiang, 323000, China.
Open Life Sci. 2025 Aug 8;20(1):20251145. doi: 10.1515/biol-2025-1145. eCollection 2025.
Determining the critical elements in melanoma stem cell growth could aid in preventing the development of malignant cancer. The purpose of this study was to illustrate how FBXO31 affects the stemness, invasion, and migratory properties of melanoma stem cells. Side population (SP) cells with tumor stem cell characteristics were sorted from A375 melanoma cells. The mRNA and protein expression levels of FBXO31 in SP cells were detected using molecular techniques. FBXO31 was then transfected into SP cells, and the proportion of CD147 (+) cells in SP cells was detected by flow cytometry. FBXO31 was also transfected into CD147 (+) cells, and their spheroid formation, migration, and invasion ability were measured. Additionally, CD147 (-) and CD147 (+) cells were inoculated into nude mice to assess the effect of FBXO31 on tumor growth and metastasis. The findings demonstrate that FBXO31 is downregulated in SP cells. Upon FBXO31 transfection, the proportion of CD147 (+) cells sorted from SP cells decreased. CD147 (+) cells exhibit higher stemness characteristics, migration, and invasion abilities than CD147 (-) cells. However, these characteristics were markedly suppressed following FBXO31 transfection in CD147 (+) cells. experiment further showed that CD147 (+) cells promoted tumor growth and metastasis, while after transfection with FBXO31, tumor proliferation and metastatic abilities were inhibited. Overall, FBXO31 inhibits the migration, invasion, and stemness characteristics of CD147 (+) melanoma stem cells.
确定黑色素瘤干细胞生长中的关键因素有助于预防恶性肿瘤的发展。本研究的目的是阐明FBXO31如何影响黑色素瘤干细胞的干性、侵袭和迁移特性。从A375黑色素瘤细胞中筛选出具有肿瘤干细胞特征的侧群（SP）细胞。采用分子技术检测SP细胞中FBXO31的mRNA和蛋白表达水平。然后将FBXO31转染到SP细胞中，通过流式细胞术检测SP细胞中CD147（+）细胞的比例。还将FBXO31转染到CD147（+）细胞中，测量其成球、迁移和侵袭能力。此外，将CD147（-）和CD147（+）细胞接种到裸鼠体内，以评估FBXO31对肿瘤生长和转移的影响。研究结果表明，FBXO31在SP细胞中表达下调。转染FBXO31后，从SP细胞中筛选出的CD147（+）细胞比例降低。CD147（+）细胞比CD147（-）细胞表现出更高的干性特征、迁移和侵袭能力。然而，在CD147（+）细胞中转染FBXO31后，这些特征明显受到抑制。实验进一步表明，CD147（+）细胞促进肿瘤生长和转移，而转染FBXO31后，肿瘤增殖和转移能力受到抑制。总体而言，FBXO31抑制CD147（+）黑色素瘤干细胞的迁移、侵袭和干性特征。
