Galal Manar M, Ismail Amira Galal, Nashaat Yousra, Hamdy Tamer M
Restorative and Dental Materials Department, Oral and Dental Research Institute, National Research Centre (NRC), Giza, Dokki, 12622, Egypt.
Endodontic Department, Faculty of Dentistry, October University, Cairo, Egypt.
J Oral Biol Craniofac Res. 2025 Jul-Aug;15(4):757-762. doi: 10.1016/j.jobcr.2025.05.002. Epub 2025 May 10.
This study is aimed at evaluating the cytotoxicity, apoptosis/necrosis, and calcium ion release of ADseal (a resin-based sealer) and two recently introduced bioceramic-based root canal sealers, which are VDW.1 seal bioceramic and Fill Root ST.
The cellular responses were studied in human periodontal ligament stem cells (hPDLSCs). Four root canal sealer extracts concentrations (100 %, 50 %, 25 %, and 12.5 %) were assessed for cell viability using the cell viability MTT essay and after 24 h, 48 h, and 7 days for all concentrations. To determine the effect of materials on apoptosis and necrosis, flow cytometry analysis was used. Calcium ion release was evaluated after 14 days of storage in distilled water using optical emission spectroscopy (ICP-OES). A two-way ANOVA was used for the statistical analysis of the acquired results.
Fill Root ST and VDW.1 seal bioceramic showed the highest amount of cell viability compared to ADseal sealer. Moreover, Fill Root ST, followed by VDW.1 seal bioceramic, demonstrated decreased cell apoptosis and necrosis. Moreover, VDW.1 seal bioceramic showed the highest calcium ion release, followed by Fill Root ST. ADseal sealers showed the lowest calcium ion release compared to bioceramic-based root canal sealers.
Based on the results of this study, in terms of cytotoxicity, apoptosis/necrosis, and remineralization ability, bioceramic-based root canal sealers were more biocompatible than ADseal resin-based sealers.
本研究旨在评估ADseal（一种树脂类封闭剂）以及两种最近推出的生物陶瓷类根管封闭剂VDW.1密封生物陶瓷和Fill Root ST的细胞毒性、凋亡/坏死情况以及钙离子释放情况。
在人牙周膜干细胞（hPDLSCs）中研究细胞反应。使用细胞活力MTT试验评估四种根管封闭剂提取物浓度（100%、50%、25%和12.5%）对细胞活力的影响，所有浓度均在24小时、48小时和7天后进行评估。为确定材料对凋亡和坏死的影响，采用流式细胞术分析。使用光发射光谱法（ICP - OES）评估在蒸馏水中储存14天后的钙离子释放情况。对获得的结果进行统计分析时采用双向方差分析。
与ADseal封闭剂相比，Fill Root ST和VDW.1密封生物陶瓷显示出最高的细胞活力。此外，Fill Root ST其次是VDW.1密封生物陶瓷，表现出细胞凋亡和坏死减少。而且，VDW.1密封生物陶瓷显示出最高的钙离子释放，其次是Fill Root ST。与生物陶瓷类根管封闭剂相比，ADseal封闭剂显示出最低的钙离子释放。
基于本研究结果，在细胞毒性、凋亡/坏死和再矿化能力方面，生物陶瓷类根管封闭剂比ADseal树脂类封闭剂具有更高的生物相容性。
