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不同封闭剂对人牙周膜干细胞细胞相容性和成骨潜能的影响：一项体外研究

Effect of Different Sealers on the Cytocompatibility and Osteogenic Potential of Human Periodontal Ligament Stem Cells: An In Vitro Study.

作者信息

Saber Shehabeldin, Raafat Shereen, Elashiry Mohamed, El-Banna Ahmed, Schäfer Edgar

机构信息

Department of Endodontics, Faculty of Dentistry, The British University in Egypt (BUE), El Sherouk City 11837, Egypt.

Centre for Innovative Dental Sciences (CIDS), Faculty of Dentistry, The British University in Egypt (BUE), El Sherouk City 11837, Egypt.

出版信息

J Clin Med. 2023 Mar 17;12(6):2344. doi: 10.3390/jcm12062344.

DOI:10.3390/jcm12062344
PMID:36983344
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10056919/
Abstract

BACKGROUND

There is tendency for unavoidable sealer extrusion in some clinical cases. This might adversely affect host stem cells and affect healing. This study aimed to investigate the effect of different sealers on the cytocompatibility and osteogenic potential of human periodontal ligament stem cells (PDLSCs).

METHODS

The cytotoxic effect of the extracted elutes of VDW.1Seal (VDW.1), Endosequence BC Sealer HiFlow (ES), GuttaFlow-2 (GF), and ADSeal (AD-S) on the PDLSCs was determined using the MTT assay. Cell proliferation and migration were assessed by the scratch wound healing assay. Osteogenic differentiation potential was assessed. Measurement of pH values and calcium ions release was performed.

RESULTS

GF had a significantly higher percentage of viable cells. The cell migration assay showed that GF demonstrated the lowest open wound area percentage. GF and AD-S showed the highest calcium nodule deposition. GF demonstrated higher ALP activity than ES. Expression of RUNX2 and OC genes was similar for all sealers, while OPG gene expression was significantly higher for VDW.1 and GF. ES and AD-S displayed the highest pH values on day 1. Calcium ion release of ES and VDW.1 was significantly the highest.

CONCLUSIONS

GuttaFlow-2 and VDW.1Seal sealers have favorable behavior toward host stem cells.

摘要

背景

在一些临床病例中，不可避免地会出现封闭剂挤出的情况。这可能会对宿主干细胞产生不利影响并影响愈合。本研究旨在探讨不同封闭剂对人牙周膜干细胞（PDLSCs）细胞相容性和成骨潜能的影响。

方法

使用MTT法测定VDW.1Seal（VDW.1）、Endosequence BC Sealer HiFlow（ES）、GuttaFlow-2（GF）和ADSeal（AD-S）提取洗脱液对PDLSCs的细胞毒性作用。通过划痕伤口愈合试验评估细胞增殖和迁移。评估成骨分化潜能。进行pH值和钙离子释放的测量。

结果

GF的活细胞百分比显著更高。细胞迁移试验表明，GF的开放伤口面积百分比最低。GF和AD-S显示出最高的钙结节沉积。GF的碱性磷酸酶（ALP）活性高于ES。所有封闭剂的RUNX2和OC基因表达相似，而VDW.1和GF的骨保护素（OPG）基因表达显著更高。ES和AD-S在第1天显示出最高的pH值。ES和VDW.1的钙离子释放显著最高。

结论

GuttaFlow-2和VDW.1Seal封闭剂对宿主干细胞具有良好的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/9fa7e0136be8/jcm-12-02344-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/2295b35a0937/jcm-12-02344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/25720288b963/jcm-12-02344-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/0f1802ae979f/jcm-12-02344-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/5fd5a58ba792/jcm-12-02344-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/0f1d180a5f38/jcm-12-02344-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/9ed1a986b92c/jcm-12-02344-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/5bc0e661c969/jcm-12-02344-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/30398c7183b8/jcm-12-02344-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/9fa7e0136be8/jcm-12-02344-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/2295b35a0937/jcm-12-02344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/25720288b963/jcm-12-02344-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/0f1802ae979f/jcm-12-02344-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/5fd5a58ba792/jcm-12-02344-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/0f1d180a5f38/jcm-12-02344-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/9ed1a986b92c/jcm-12-02344-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/5bc0e661c969/jcm-12-02344-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/30398c7183b8/jcm-12-02344-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a197/10056919/9fa7e0136be8/jcm-12-02344-g009.jpg

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