Matsumoto Tomoko, Tujii Nukumi, Shimomura Daiki, Kouno Aya, Suzuki Takeshi, Shinohara Sho, Arai Nobuo, Kurono Hiroshi, Kumano Osamu, Kamioka Mikio
Department of Clinical Laboratory Science, Tenri University, Tenri City, Nara, Japan.
Department of Clinical Laboratory Science, Tenri Health Care University, Tenri City, Nara, Japan.
PLoS One. 2025 Aug 18;20(8):e0320111. doi: 10.1371/journal.pone.0320111. eCollection 2025.
Clot-fibrinolysis waveform analysis (CFWA) is an assay used to simultaneously evaluate coagulation and fibrinolysis reactions. Although the assay detected the reaction via transmittance changes, there was no evidence that the transmittance changes indicated reactions. This study aimed to demonstrate that transmittance changes indicate coagulation and fibrinolysis reactions by detecting relative markers. CFWA was conducted using activated partial thromboplastin time (APTT) reagent and CaCl2 solution with tissue-plasminogen activator (tPA); transmittance changes were monitored, and the first derivative curve was analyzed in pooled normal plasma (PNP) and factors V, VIII, IX, X, and XI-deficient plasma samples. The samples during the coagulation and fibrinolysis reactions were prepared by adding the reaction stop solution, fibrin monomer complex (FMC), fibrin/fibrinogen degradation products (FDP), D-dimer and plasmin-α2 plasmin inhibitor complex (PAP) were measured to compare the waveform with the tendencies of these markers. The fibrinolysis markers FDP, D-dimer, and PAP increased in all samples as the reaction time increased. In FMC, the value increased during the coagulation phase, decreased at the end of the phase, and increased again during the fibrinolysis phase. FMC, FDP, and D-dimers were generated from fibrin/fibrinogen in the CFWA assay, indicating that the assay reflects coagulation and fibrinolysis reactions by monitoring transmittance.
凝块纤维蛋白溶解波形分析（CFWA）是一种用于同时评估凝血和纤维蛋白溶解反应的检测方法。尽管该检测方法通过透光率变化来检测反应，但没有证据表明透光率变化表明了反应。本研究旨在通过检测相关标志物来证明透光率变化表明凝血和纤维蛋白溶解反应。使用活化部分凝血活酶时间（APTT）试剂和氯化钙溶液以及组织型纤溶酶原激活剂（tPA）进行CFWA；监测透光率变化，并对混合正常血浆（PNP）以及V、VIII、IX、X和XI因子缺乏的血浆样本中的一阶导数曲线进行分析。通过添加反应终止溶液来制备凝血和纤维蛋白溶解反应期间的样本，测量纤维蛋白单体复合物（FMC）、纤维蛋白/纤维蛋白原降解产物（FDP）、D-二聚体和纤溶酶-α2纤溶酶抑制剂复合物（PAP），以将波形与这些标志物的趋势进行比较。随着反应时间的增加，所有样本中的纤维蛋白溶解标志物FDP、D-二聚体和PAP均增加。在FMC中，其值在凝血阶段增加，在该阶段结束时下降，并在纤维蛋白溶解阶段再次增加。在CFWA检测中，FMC、FDP和D-二聚体是由纤维蛋白/纤维蛋白原产生的，这表明该检测方法通过监测透光率反映凝血和纤维蛋白溶解反应。
