Joung Su-Hyeon, Lee Yu-Si, Kim Byeong Joon, Park Yongchjun, Kim Seung Hwan, Kim Soon Han, Joo Insun, An Eun Sook
Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Republic of Korea.
J Microbiol Biotechnol. 2025 Aug 18;35:e2504003. doi: 10.4014/jmb.2504.04003.
Most positive control strains in microbial assays are sourced from abroad. ATCC 14579 is used as the positive control strain in microbial assays described in the Manual for the Detection of Foodborne Pathogens at Outbreaks and the FDA's Bacteriological Analytical Manual method. This study aimed to identify a domestically isolated strain from the Ministry of Food and Drug Safety (MFDS) to replace ATCC 14579. A total of 323 strains from the MFDS were collected, and gene-targeted polymerase chain reaction and biochemical tests were performed to characterize candidate strains. Sixty-one candidate strains showing identical biochemical and molecular characteristics to ATCC 14579 were further subjected to whole-genome sequencing. Comparative genetic analyses using MLST, SNP distance, and OrthoANI revealed that MFDS 1004972 shared the same sequence type, exhibited SNP distance of fewer than 10 SNPs, and showed 100% ANI with ATCC 14579. Therefore, MFDS 1004972 is proposed as a suitable domestic alternative to the imported strain.
微生物检测中的大多数阳性对照菌株都来自国外。美国典型培养物保藏中心（ATCC）14579被用作《食源性疾病暴发检测手册》和美国食品药品监督管理局（FDA）《细菌学分析手册》方法中所述微生物检测的阳性对照菌株。本研究旨在从韩国食品药品安全部（MFDS）分离出一株国内菌株来替代ATCC 14579。共收集了323株来自MFDS的菌株，并进行了基因靶向聚合酶链反应和生化试验以鉴定候选菌株。对61株与ATCC 14579具有相同生化和分子特征的候选菌株进一步进行全基因组测序。使用多位点序列分型（MLST）、单核苷酸多态性（SNP）距离和平均核苷酸一致性（OrthoANI）进行的比较遗传分析表明，MFDS 1004972与ATCC 14579具有相同的序列类型，SNP距离少于10个SNP，且ANI为100%。因此，建议将MFDS 1004972作为进口菌株的合适国内替代菌株。
