Lv YongBo, Zhu Dashuai, Li Junlang, Li Yuan, Lu Chao, Bi Jianing, Huang Ke, Hu Shiqi, Liu Shuo, Yan Na, Cheng Ke, Wang Juan
Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China.
Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, USA.
Nat Commun. 2025 Aug 18;16(1):7678. doi: 10.1038/s41467-025-62020-5.
Ischemic injury and reperfusion injury collectively determine the total infarct size, a major prognostic factor following myocardial infarction (MI). Therefore, addressing both ischemic and reperfusion stages could substantially reduce infarct size and improve clinical outcomes. In this study, we develop a two-component therapeutic system from different parts of Glycyrrhiza: a functional hydrogel made from glycyrrhizic acid extracted from the stem and root, and nanosized chloroplast units (NCUs) derived from leaves. The hydrogel demonstrates therapeutic effects during both hypoxia and reoxygenation stages in vitro, while the photosynthetic NCUs alleviate hypoxia injury by providing ATP and NADPH under illumination. Subsequent in vivo study reveals the most significant therapeutic effect in the combination group (NCU plus hydrogel), which effectively treats both ischemic and reperfusion stages. Our study highlights the cross-species application of plant photosynthetic mechanisms in MI treatment and confirms that simultaneous treatment of ischemia and reperfusion is more effective than treating either stage alone, offering a promising therapeutic strategy for MI.
缺血性损伤和再灌注损伤共同决定了总的梗死面积，这是心肌梗死（MI）后一个主要的预后因素。因此，针对缺血和再灌注阶段都进行干预，可显著减小梗死面积并改善临床结局。在本研究中，我们从甘草的不同部位开发了一种双组分治疗系统：一种由从茎和根中提取的甘草酸制成的功能性水凝胶，以及源自叶片的纳米级叶绿体单元（NCUs）。该水凝胶在体外的缺氧和复氧阶段均显示出治疗效果，而光合NCUs通过在光照下提供ATP和NADPH来减轻缺氧损伤。随后的体内研究表明，联合组（NCU加水凝胶）具有最显著的治疗效果，可有效治疗缺血和再灌注两个阶段。我们的研究突出了植物光合机制在MI治疗中的跨物种应用，并证实同时治疗缺血和再灌注比单独治疗任一阶段更有效，为MI提供了一种有前景的治疗策略。
