Aceto Serena, Perrini Stefano, Varone Marianna, Lucibelli Francesca, Volpe Gennaro, Di Lillo Paola, Carfora Angela, Mazzucchiello Sarah Maria, Saccone Giuseppe, Salvemini Marco
Department of Biology, University of Naples Federico II, Naples, Italy.
Methods Mol Biol. 2025;2935:273-298. doi: 10.1007/978-1-0716-4583-3_12.
Sex-specific transcripts are RNA molecules expressed predominantly or exclusively in one sex, providing insights into molecular and physiological differences between males and females. This knowledge underpins the development of precise and efficient genetic sexing methods applicable in various contexts. In agriculture and livestock management, early sex determination could enhance resource management and productivity. In ecology and conservation, genetic sexing informs population monitoring and species management. In applied entomology, it could improve biological control strategies, such as the sterile insect technique. Here, we describe a bioinformatic framework to identify sex-specific transcripts using RNA-seq sequencing data in eukaryotic species with or without a sequenced reference genome.
性别特异性转录本是主要或仅在一种性别中表达的RNA分子，有助于深入了解雄性和雌性之间的分子和生理差异。这些知识为开发适用于各种情况的精确高效的遗传性别鉴定方法奠定了基础。在农业和畜牧管理中，早期性别鉴定可以加强资源管理并提高生产力。在生态学和保护领域，遗传性别鉴定为种群监测和物种管理提供信息。在应用昆虫学中，它可以改进生物防治策略，如不育昆虫技术。在此，我们描述了一个生物信息学框架，用于在有或没有已测序参考基因组的真核生物物种中，使用RNA测序数据鉴定性别特异性转录本。
