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马立克氏病病毒的pUL47被膜蛋白与p32/C1QBP相互作用以促进水平传播。

The pUL47 tegument protein of Marek's Disease Virus interacts with p32/C1QBP to promote horizontal transmission.

作者信息

Durand Mallorie, Chuard Aurélien, Rémy Sylvie, Courvoisier-Guyader Katia, Leclercq Sébastien, Pichon Julien, Denesvre Caroline, Pasdeloup David

机构信息

Laboratory of Biology of Avian Viruses, INRAE-Université de Tours, UMR1282 ISP, Nouzilly, France.

Laboratory of Genomic Plasticity, Biodiversity, and Antimicrobial Resistance, INRAE-Université de Tours, UMR1282 ISP, Nouzilly, France.

出版信息

PLoS Pathog. 2025 Aug 19;21(8):e1013434. doi: 10.1371/journal.ppat.1013434. eCollection 2025 Aug.

DOI:10.1371/journal.ppat.1013434
PMID:40828852
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12380274/
Abstract

Inter-individual transmission is an essential part of viruses' life cycle, yet the molecular basis of transmission remains elusive. Using Marek's Disease Virus (MDV), a deadly and contagious herpesvirus of chickens that is transmitted by inhalation of contaminated dander, we previously demonstrated that tegument protein pUL47 was necessary for horizontal transmission in the natural host while being dispensable for pathogenesis and replication. We showed that pUL47 was also necessary for the correct splicing and expression of UL44 transcripts, which encode glycoprotein gC, another viral protein known as essential for MDV's transmission. Here, with the aim of characterizing the molecular basis for the role of pUL47 in transmission, we identify the cellular protein p32/C1QBP/gC1qR, a regulator of mRNA splicing, as a binding partner. We demonstrate that this interaction is necessary for transmission while being dispensable for the correct splicing and expression of UL44 transcripts, thus uncoupling the role of pUL47 in transmission from gC. These results provide a deeper molecular understanding of the natural transmission of a Herpesvirus.

摘要

个体间传播是病毒生命周期的重要组成部分，然而传播的分子基础仍然难以捉摸。利用马立克氏病病毒（MDV），一种通过吸入受污染的皮屑传播的鸡的致命性和传染性疱疹病毒，我们之前证明，被膜蛋白pUL47对于自然宿主中的水平传播是必需的，而对于发病机制和复制则是可有可无的。我们表明，pUL47对于UL44转录本的正确剪接和表达也是必需的，UL44转录本编码糖蛋白gC，这是另一种已知对MDV传播至关重要的病毒蛋白。在这里，为了阐明pUL47在传播中作用的分子基础，我们鉴定了细胞蛋白p32/C1QBP/gC1qR，一种mRNA剪接调节因子，作为其结合伴侣。我们证明这种相互作用对于传播是必需的，而对于UL44转录本的正确剪接和表达则是可有可无的，从而将pUL47在传播中的作用与gC分离开来。这些结果为疱疹病毒的自然传播提供了更深入的分子理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/5ffca18ccb96/ppat.1013434.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/4f9260b4e2f0/ppat.1013434.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/19e7e1797542/ppat.1013434.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/05b3fbfcf740/ppat.1013434.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/3208db660b8d/ppat.1013434.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/48ec2f0a6c9d/ppat.1013434.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/2e17cdc7b0ac/ppat.1013434.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/34093316824b/ppat.1013434.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/7fd93bb2be0c/ppat.1013434.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/6379ee24ece2/ppat.1013434.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/5ffca18ccb96/ppat.1013434.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/4f9260b4e2f0/ppat.1013434.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/19e7e1797542/ppat.1013434.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/05b3fbfcf740/ppat.1013434.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/3208db660b8d/ppat.1013434.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/48ec2f0a6c9d/ppat.1013434.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/2e17cdc7b0ac/ppat.1013434.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/34093316824b/ppat.1013434.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/7fd93bb2be0c/ppat.1013434.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/6379ee24ece2/ppat.1013434.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1558/12380274/5ffca18ccb96/ppat.1013434.g010.jpg

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