Staufer Theresa, Kopatz Verena, Pradel Alice, Brodie Tess, Kuhrwahl Robert, Stroka Deborah, Wallner Julia, Kenner Lukas, Pichler Verena, Grüner Florian, Mitrano Denise M
University of Hamburg, Fachbereich Physik, and Center for Free-Electron Laser Science (CFEL), Hamburg, Germany.
Department of Pathology, Medical University of Vienna, Vienna, Austria.
Commun Biol. 2025 Aug 19;8(1):1247. doi: 10.1038/s42003-025-08709-1.
Contamination of plastic particles in environmental and biological systems raises concerns regarding their potential negative impacts. Human exposure to microplastics (MPs) and nanoplastics (NPs) is increasing; however, some potential adverse health effects might remain unclear, due to analytical challenges in detecting trace concentrations. To address this, we propose a workflow for NPs assessment in biological samples combining three complimentary methods, namely inductively coupled plasma mass spectrometry (ICP-MS), X-ray fluorescence imaging (XFI), and imaging mass cytometry (IMC) to detect palladium-doped NPs (Pd-NPs). This approach was used to quantify the temporal distribution and accumulation of Pd-NPs in mouse models under different experimental conditions, dosages, and time frames. Acute exposure showed a clear particle excretion from the gastrointestinal tract into feces, while subchronic exposure led to tissue accumulation. This workflow enhances our ability to analyze and study NP uptake and biodistribution mechanisms down to the nanoscale in complex biological samples.
环境和生物系统中塑料颗粒的污染引发了人们对其潜在负面影响的担忧。人类对微塑料（MPs）和纳米塑料（NPs）的接触正在增加；然而，由于检测痕量浓度存在分析挑战，一些潜在的健康不利影响可能仍不明确。为了解决这一问题，我们提出了一种用于生物样品中纳米塑料评估的工作流程，该流程结合了三种互补方法，即电感耦合等离子体质谱法（ICP-MS）、X射线荧光成像（XFI）和成像质谱流式细胞术（IMC），以检测钯掺杂纳米塑料（Pd-NPs）。该方法用于量化不同实验条件、剂量和时间范围内小鼠模型中Pd-NPs的时间分布和积累情况。急性暴露显示颗粒从胃肠道明显排泄到粪便中，而亚慢性暴露则导致组织积累。这种工作流程增强了我们在复杂生物样品中分析和研究纳米塑料摄取及生物分布机制直至纳米尺度的能力。
