Fachbereich Physik, Universität Hamburg, 22761, Hamburg, Germany.
Center for Free-Electron Laser Science (CFEL), 22761, Hamburg, Germany.
Sci Rep. 2023 Jul 17;13(1):11505. doi: 10.1038/s41598-023-38536-5.
The infiltration of immune cells into sites of inflammation is one key feature of immune mediated inflammatory diseases. A detailed assessment of the in vivo dynamics of relevant cell subtypes could booster the understanding of this disease and the development of novel therapies. We show in detail how advanced X-ray fluorescence imaging enables such quantitative in vivo cell tracking, offering solutions that could pave the way beyond what other imaging modalities provide today. The key for this achievement is a detailed study of the spectral background contribution from multiple Compton scattering in a mouse-scaled object when this is scanned with a monochromatic pencil X-ray beam from a synchrotron. Under optimal conditions, the detection sensitivity is sufficient for detecting local accumulations of the labelled immune cells, hence providing experimental demonstration of in vivo immune cell tracking in mice.
免疫细胞浸润到炎症部位是免疫介导的炎症性疾病的一个关键特征。对相关细胞亚型的体内动力学进行详细评估，可以增进对这种疾病的理解，并开发新的治疗方法。我们详细展示了先进的 X 射线荧光成像如何实现这种定量的体内细胞跟踪，提供的解决方案可能超越其他成像模式目前所能提供的。这一成就的关键是在对小鼠大小的物体进行同步加速器单色铅笔 X 射线扫描时，对多次康普顿散射的光谱背景贡献进行详细研究。在最佳条件下，检测灵敏度足以检测到标记免疫细胞的局部积聚，从而在小鼠体内进行了免疫细胞跟踪的实验演示。
