Mohsen Abdelrahman A, El-Mahdy Taghrid S, Emara Mohamed, Salim Samar A
Department of Microbiology and Immunology, Faculty of pharmacy, Helwan university-Ain Helwan, Helwan, 11795, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Cairo, Egypt.
J Biol Eng. 2025 Aug 19;19(1):76. doi: 10.1186/s13036-025-00549-1.
The increasing resistance of bacteria to conventional antibiotics poses a significant health challenge. Innovative strategies, such as combining antibiotics with agents like quorum sensing inhibitors (QSIs), have been developed to combat this issue. QSIs enhance antibiotic efficacy without inhibiting bacterial growth, minimizing the risk of resistance.
Evaluate the combined effect of Sitagliptin (STG) as a QSI with Meropenem (MER), fabricate drug-loaded Polyvinyl Alcohol (PVA) nanofibers, and investigate their antimicrobial activity against standard Pseudomonas aeruginosa (PAO1) and carbapenem-resistant P. aeruginosa (CRPA).
The combinatorial effect was assessed using a checkerboard assay. PVA/STG, PVA/MER, and PVA/STG/MER nanofibers were fabricated with varying concentrations of STG (2, 4, 8 mg/mL) and MER (5, 7, 9 mg/mL) via electrospinning. Characterization was performed using FTIR, XRD, and SEM techniques.
STG significantly reduced the minimum inhibitory concentration (MIC) of MER. The 1:2 STG to MER ratio exhibited the highest antimicrobial activity, achieving a comparable zone of inhibition to the highest concentration of MER while utilizing nearly half the amount of MER. The stability of the loaded scaffolds was maintained over three months at 2-8 °C.
Our results underscore the successful fabrication of nanofiber scaffolds and the effectiveness of STG and MER-loaded nanofibers as promising wound dressings for cutaneous P. aeruginosa infections. This study highlights the potential of our innovative nanofiber system to enhance treatment efficacy against multidrug-resistant bacteria, offering a personalized and rapid response wound dressing solution for medical professionals. Ultimately, it shows promise to improve patient recovery and quality of life while minimizing systemic side effects.
细菌对传统抗生素的耐药性不断增加，这对健康构成了重大挑战。人们已开发出创新策略，如将抗生素与群体感应抑制剂（QSIs）等药物联合使用，以应对这一问题。QSIs可增强抗生素疗效，同时不抑制细菌生长，从而将耐药风险降至最低。
评估西他列汀（STG）作为一种QSIs与美罗培南（MER）联合使用的效果，制备载药聚乙烯醇（PVA）纳米纤维，并研究其对标准铜绿假单胞菌（PAO1）和耐碳青霉烯类铜绿假单胞菌（CRPA）的抗菌活性。
采用棋盘法评估联合效果。通过静电纺丝制备不同浓度STG（2、4、8mg/mL）和美罗培南（5、7、9mg/mL）的PVA/STG、PVA/MER和PVA/STG/MER纳米纤维。使用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和扫描电子显微镜（SEM）技术进行表征。
STG显著降低了MER的最低抑菌浓度（MIC）。STG与MER的1:2比例表现出最高的抗菌活性，在使用近一半MER量的情况下，抑菌圈与最高浓度的MER相当。负载支架在2-8°C下可保持三个月的稳定性。
我们的结果强调了纳米纤维支架的成功制备，以及载有STG和MER的纳米纤维作为治疗皮肤铜绿假单胞菌感染的有前景的伤口敷料的有效性。本研究突出了我们创新的纳米纤维系统在增强对多重耐药菌治疗效果方面的潜力，为医疗专业人员提供了一种个性化且快速响应的伤口敷料解决方案。最终，它有望改善患者的康复情况和生活质量，同时将全身副作用降至最低。
