GTSE1-expressed osteoblastic cells facilitate formation of pro-metastatic tumor microenvironment in osteosarcoma.

Understanding metastatic osteosarcoma relies on defining the complexity of cell types, their associated molecular profiles, and interactions among cells in the tumor microenvironment. Here, we integrated single-cell and bulk gene expression datasets and revealed that metastatic lesions were highly enriched for osteoblasts (OB). Under the regulation of E2F family members, OB cells harbored enhanced proliferation activity and high differentiation potential. Augmentation of enhanced the abilities of cell migration and invasion, while silencing of impaired the abilities in human OB cell lines. Furthermore, cellular communication analysis showed the cross-talk between OB cells and CD8 T cells in metastasis was achieved through the -(-) pair. Spatial transcriptomic data revealed that - and -/ showed a higher positive correlation in undifferentiated pleomorphic sarcoma than leiomyosarcoma. Correlation analysis unveiled that OB cells and monocytes were the negatively correlated populations at the single-cell level, a finding validated in 4 independent osteosarcoma datasets comprising 226 samples. Our findings suggest that overexpression serves as a potential biomarker for metastasis in osteosarcoma and provides a promising strategy to prevent metastasis by targeting OB cells.