Tchernitchin A N, Barrera J, Arroyo P, Mena M A, Vilches K, Grunert G
Agents Actions. 1985 Oct;17(1):60-6. doi: 10.1007/BF01966683.
The degranulatory activity of estradiol was tested on blood eosinophil leukocytes in vivo and in vitro. Treatment of adult ovariectomized rats with 30 micrograms estradiol/100 g body wt. increased the percentage of degranulated eosinophils from 10% to about 70% at 6 or 24 h after treatment, and increased the rate of in vitro eosinophil degranulation of those eosinophils that were non-degranulated at the time of blood sample collection. The addition of estradiol to blood samples from untreated ovariectomized rats (0.05 micrograms/ml) increased the percentage of eosinophils degranulated in vitro at 1 h of incubation from 20% to near 60%. It is suggested that estrogen acts directly on the eosinophils, probably via specific hormone receptors. Considering the hypothesis that eosinophils are involved in the mediation of a group of responses to estrogen in the uterus, it is proposed that the hormone-induced degranulation of these cells may have physiological implications in the regulation of the intensity and duration of the eosinophil-mediated responses. In addition, estrogen-induced eosinophil degranulation may interfere with the action of eosinophils that have migrated to other target organs (lymphoid organs, etc.) under the action of other agents (glucocorticoid hormones, histamine, etc.) or conditions (immune reactions, etc.).
在体内和体外对血液嗜酸性粒细胞进行了雌二醇脱颗粒活性测试。用30微克雌二醇/100克体重对成年去卵巢大鼠进行处理，在处理后6或24小时，脱颗粒嗜酸性粒细胞的百分比从10%增加到约70%，并且增加了在采集血样时未脱颗粒的嗜酸性粒细胞的体外脱颗粒速率。向未处理的去卵巢大鼠的血样中添加雌二醇（0.05微克/毫升），在孵育1小时时，体外脱颗粒的嗜酸性粒细胞百分比从20%增加到近60%。提示雌激素可能通过特异性激素受体直接作用于嗜酸性粒细胞。考虑到嗜酸性粒细胞参与子宫中一组雌激素反应介导的假说，有人提出激素诱导的这些细胞脱颗粒可能在调节嗜酸性粒细胞介导反应的强度和持续时间方面具有生理意义。此外，雌激素诱导的嗜酸性粒细胞脱颗粒可能会干扰在其他因子（糖皮质激素、组胺等）作用下或在其他条件（免疫反应等）下迁移到其他靶器官（淋巴器官等）的嗜酸性粒细胞的作用。
