枯草芽孢杆菌糖化型α-淀粉酶催化苯基β-麦芽糖苷的水解反应
Hydrolysis of phenyl beta-maltoside catalyzed by saccharifying alpha-amylase from Bacillus subtilis.
作者信息
Ishikura K, Nitta Y, Watanabe T
出版信息
J Biochem. 1977 May;81(5):1187-92.
PMID:408329
Abstract
- The hydrolytic reaction of phenyl beta-maltoside catalyzed by saccharifying alpha-amylase [EC 3.2.1.1] of Bacillus subtilis was studied at 25 degrees C and pH 5.4, by measuring the total reducing power and the amount of phenol liberated, and by thin layer chromatography. 2. The enzyme hydrolyzed phenyl beta-maltoside at the glucosidic linkage between the two glucose residues to form D-glucose and phenyl beta-D-glucoside. Besides these products, maltose, maltotriose, and phenyl beta-maltotrioside were also observed as reaction products. The identification of phenyl beta-maltotrioside is described in detail. The formation of these products was attributed to the transglycosylation reaction of the enzyme. The time course of reaction as followed by reducing power measurement showed an induction period of several minutes.
摘要
- 在25℃和pH 5.4条件下，通过测定总还原能力、释放的苯酚量以及采用薄层色谱法，研究了枯草芽孢杆菌糖化α-淀粉酶[EC 3.2.1.1]催化苯基β-麦芽糖苷的水解反应。2. 该酶在两个葡萄糖残基之间的糖苷键处水解苯基β-麦芽糖苷，形成D-葡萄糖和苯基β-D-葡萄糖苷。除了这些产物外，还观察到麦芽糖、麦芽三糖和苯基β-麦芽三糖苷作为反应产物。详细描述了苯基β-麦芽三糖苷的鉴定过程。这些产物的形成归因于该酶的转糖基化反应。通过还原能力测量跟踪的反应时间进程显示有数分钟的诱导期。
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