Negrín-Santamaría Isaac, Trujillo-Rodríguez María J, Moyano Encarnación, Ayala Juan H, Zuloaga Olatz, Pasán Jorge, Pino Verónica, Ayala-Cabrera Juan F
Laboratorio de Materiales para Análisis Químico (MAT4LL), Departamento de Química, Unidad Departamental de Química Analítica, Universidad de La Laguna (ULL), San Cristóbal de La Laguna 38108, Spain.
Unidad de Investigación de Bioanalítica y Medioambiente, Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias (IUETSPC), ULL, San Cristóbal de La Laguna 38108, Spain.
Anal Chem. 2025 Sep 2;97(34):18621-18629. doi: 10.1021/acs.analchem.5c02782. Epub 2025 Aug 20.
Ambient ionization mass spectrometry (AIMS) techniques have become an emerging approach over the last years due to their simplicity, permitting high-throughput sample analysis by directly coupling with mass spectrometry while ensuring short analysis times. Among AIMS techniques, coated-blade spray (CBS) has stood out, as it ensures a significant enhancement of overall sensitivity, undoubtedly useful for human biofluid analysis. In parallel, the incorporation of advanced smart materials, such as metal-organic frameworks (MOFs), into analytical devices is increasing due to their outstanding ability to efficiently trap target analytes, such as industrial chemicals with diverse functionalities and polarities. This study integrates neat MOFs in CBS devices, without the need for any composite or additional materials, through a simple and mild strategy and shows their use in the determination of xenobiotics present in human urine samples. Moreover, a suspect screening workflow by high-resolution mass spectrometry (HRMS) has been developed for the first time to extend the chemical coverage of AIMS techniques. This simultaneous approach ensures a proper analytical quality performance, achieving limits of quantification (LOQs) down to 0.1 ng·mL despite requiring only 8 min for the entire procedure.
近年来，常压电离质谱（AIMS）技术因其操作简便而成为一种新兴方法，它通过与质谱直接联用实现高通量样品分析，同时保证了较短的分析时间。在AIMS技术中，涂层刀片喷雾（CBS）脱颖而出，因为它能显著提高整体灵敏度，这无疑对人体生物流体分析很有用。与此同时，先进的智能材料，如金属有机框架（MOF），因其能高效捕获目标分析物（如具有不同功能和极性的工业化学品）的出色能力，在分析设备中的应用越来越多。本研究通过一种简单温和的策略，将纯净的MOF集成到CBS设备中，无需任何复合材料或额外材料，并展示了其在测定人尿样中存在的外源性物质方面的应用。此外，首次开发了一种通过高分辨率质谱（HRMS）进行可疑物筛查的工作流程，以扩展AIMS技术的化学覆盖范围。这种同步方法确保了良好的分析质量性能，尽管整个过程仅需8分钟，但定量限（LOQ）低至0.1 ng·mL。
