College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
Anal Chem. 2023 Jul 25;95(29):10984-10991. doi: 10.1021/acs.analchem.3c01098. Epub 2023 Jul 14.
Ambient mass spectrometry (AMS) allows direct analysis of various raw food samples with minimal or no sample pretreatment, but the trace analytes in complex food samples still have problems with limitations. In this work, we developed a platform based on coated stainless steel sheet spray mass spectrometry for fast, in situ, high-throughput, and high selectivity multiresidue analysis of fluoroquinolone drugs (FQs). The sensitivity of the platform was enhanced via coupling broad-spectrum antibodies against FQs to graphene oxide coated blade spray (CBS)-MS through a streptavidin-biotin (SA-biotin) interaction. The prepared platform had sufficient loading capacity for SA (1.37 mg/piece) and the antibody (84.8 μg/piece), which is greater than that of physical mixing and the EDC/NHS covalent coupling strategy. With simplified sample pretreatment, this platform demonstrated comparable sensitivity to high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) (0.08-0.16 ng/mL in phosphate-buffered saline and 0.21-0.32 ng/mL in diluted milk). Meanwhile, compared with HPLC-MS/MS, the method is rapid (enrichment: 10 min, detection: <1 min) and acceptable recoveries (81.94-102.08%) can be obtained. The presence of analytes can be monitored by MS/MS spectra, and multiple analytes can be measured simultaneously in a single assay. This study is expected to provide a powerful and portable tool for rapid laboratory analysis and reliable screening in the field.
环境基质辅助激光解析电离飞行时间质谱（AMS）允许直接分析各种原始食品样品，只需进行最小限度或无需样品预处理，但在分析复杂食品样品中的痕量分析物时仍存在局限性。在这项工作中，我们开发了一种基于涂覆不锈钢片喷雾质谱的平台，用于快速、原位、高通量和高选择性地分析氟喹诺酮类药物（FQs）的多残留。通过将广谱针对 FQs 的抗体与氧化石墨烯涂覆的刀片喷雾（CBS）-MS 通过链霉亲和素-生物素（SA-生物素）相互作用偶联，提高了平台的灵敏度。该平台具有足够的 SA（1.37 毫克/片）和抗体（84.8 微克/片）的负载能力，大于物理混合和 EDC/NHS 共价偶联策略。通过简化样品预处理，该平台与高效液相色谱-质谱联用（HPLC-MS/MS）相比具有相当的灵敏度（磷酸盐缓冲液中为 0.08-0.16 ng/mL，稀释牛奶中为 0.21-0.32 ng/mL）。同时，与 HPLC-MS/MS 相比，该方法快速（富集：10 min，检测：<1 min），可获得可接受的回收率（81.94-102.08%）。可以通过 MS/MS 谱监测分析物的存在，并在单次测定中同时测量多种分析物。这项研究有望为快速实验室分析和现场可靠筛选提供强大而便携的工具。
