Ohyama K, Kaneko I, Yamakawa T, Watanabe T
J Biochem. 1977 May;81(5):1571-4.
Two enzymatically active forms of valyl-tRNA synthetase [EC 6.1.1.9] were found in the cells of Bacillus subtilis. The aminoacylation activities of the two forms were altered during the sporulation of B. subtilis. The tRNA'S acylated by these enzymes were analyzed by methylated albumin-Kieselguhr column chromatography.
在枯草芽孢杆菌细胞中发现了两种具有酶活性的缬氨酰 - tRNA合成酶[EC 6.1.1.9]。在枯草芽孢杆菌的孢子形成过程中,这两种形式的氨酰化活性发生了改变。通过甲基化白蛋白 - 硅藻土柱色谱法分析了被这些酶酰化的tRNA。