Tatarek-Nossol Marianna, Antonin Wolfram
Institute of Biochemistry and Molecular Cell Biology, Medical School, RWTH Aachen University, Aachen, Germany.
Methods Mol Biol. 2025;2958:225-237. doi: 10.1007/978-1-0716-4714-1_15.
Many nucleoporins interact with the pore membrane, often via amphipathic helices. Liposome floatation assays are convenient and flexible assays to analyze these protein-membrane interactions and their dependence on specific lipid composition and membrane curvature. We outline here our protocols for the generation of different-sized small unilamellar liposomes of defined lipid compositions and describe how these are employed in floatation assays.
许多核孔蛋白通常通过两亲性螺旋与孔膜相互作用。脂质体漂浮测定法是分析这些蛋白质-膜相互作用及其对特定脂质组成和膜曲率依赖性的便捷且灵活的测定方法。我们在此概述了生成具有特定脂质组成的不同大小的小单层脂质体的方案，并描述了如何将其用于漂浮测定。
