研究苯并[a]芘从颗粒物洗脱到生物膜中的体外技术及其在木炉颗粒物中的应用。

In vitro technique to study elution of benzo[a]pyrene from particulates into biomembranes with application to woodstove particulates.

作者信息

Bevan D R, Yonda N T

出版信息

Anal Biochem. 1985 Oct;150(1):105-10. doi: 10.1016/0003-2697(85)90446-4.

Abstract

A method to study elution of benzo[a]pyrene (B[a]P) from carbonaceous particulates into phospholipid vesicles composed of dimyristoyl phosphatidylcholine (DMPC) was developed. Recoveries of B[a]P at each step of the procedure were quantified, with overall recoveries being 81%. Application of the technique to elution of B[a]P from woodstove particulate revealed that 25% of B[a]P associated with the particulate was eluted into DMPC vesicles in 18 h. From data obtained, it also was possible to evaluate elution of another polycyclic aromatic hydrocarbon (PAH), benzo[k]fluoranthene (B[k]F). Of the total B[k]F associated with woodstove particulate, 68% was eluted. This technique provides a convenient method for evaluating the potential bioavailability of PAH associated with other carbonaceous particulates which occur in the environment.

摘要

开发了一种研究苯并[a]芘（B[a]P）从碳质颗粒洗脱到由二肉豆蔻酰磷脂酰胆碱（DMPC）组成的磷脂囊泡中的方法。对该程序每个步骤中B[a]P的回收率进行了量化，总体回收率为81%。将该技术应用于从木炉颗粒中洗脱B[a]P，结果表明，与颗粒相关的25%的B[a]P在18小时内被洗脱到DMPC囊泡中。根据获得的数据，还可以评估另一种多环芳烃（PAH）苯并[k]荧蒽（B[k]F）的洗脱情况。与木炉颗粒相关的总B[k]F中有68%被洗脱。该技术为评估与环境中出现的其他碳质颗粒相关的PAH的潜在生物可利用性提供了一种便捷方法。