Beauregard G, Potier M
Anal Biochem. 1985 Oct;150(1):117-20. doi: 10.1016/0003-2697(85)90448-8.
The radiation inactivation method allows determination of the relative molecular mass (Mr) of proteins by exposure to high doses of ionizing radiation. The analysis by target theory of biological activity decay curves yields the size of the protein. A correction factor for Mr has been routinely used in the literature when irradiation is conducted at low temperature. Since the radiation inactivation of proteins is affected by temperature, we propose a general equation which relates Mr of a protein to D37,t, the dose in megarads at a given temperature t (in degree C) where 37% of its initial biological activity remains log Mr = 5.89 - log D37,t - 0.0028t. It is concluded that temperature affects the amount of absorbed radiation energy required to inactivate 1 mol of protein.
辐射失活法可通过让蛋白质暴露于高剂量电离辐射来测定其相对分子质量（Mr）。通过对生物活性衰减曲线进行靶理论分析可得出蛋白质的大小。当在低温下进行辐照时，文献中通常会使用一个Mr校正因子。由于蛋白质的辐射失活受温度影响，我们提出了一个通用方程，该方程将蛋白质的Mr与D37,t相关联，D37,t是在给定温度t（摄氏度）下剩余37%初始生物活性时的剂量（以兆拉德为单位），log Mr = 5.89 - log D37,t - 0.0028t。得出的结论是，温度会影响使1摩尔蛋白质失活所需吸收的辐射能量。
