Becker A B, Chung K F, McDonald D M, Lazarus S C, Frick O L, Gold W M
Anat Rec. 1985 Dec;213(4):477-80, 530-1. doi: 10.1002/ar.1092130402.
The degree of metachromasia of mast cell granules is known to vary with the type of tissue fixation and among different tissues and species. The present study sought to determine whether mast cells in dog skin are heterogeneous with respect to fixation and staining properties. We performed skin biopsies in six anesthetized, atopic dogs and one mongrel dog. One biopsy was fixed in formalin and a second, from a parallel abdominal site, was fixed in basic lead acetate (Mota's solution). Adjacent sections from each biopsy were stained with alcian blue (1%, pH 0.5) or for chloroacetate esterase activity. In alcian blue-stained sections, one-third fewer mast cells were detected in skin fixed in formalin (1,836 +/- 454 mast cells/mm3, mean +/- SEM) than in skin fixed in basic lead acetate (2,684 +/- 527 mast cells/mm3) (P less than 0.05). The chloroacetate esterase reaction detected the larger number of mast cells regardless of the fixative used. We conclude that mast cell heterogeneity, as demonstrated by metachromatic staining following different types of tissue fixation, exists in dog skin. "Typical" mast cells stain with alcian blue regardless of fixation; however, "atypical" mast cells exhibit metachromasia only after fixation in basic lead acetate. Both the typical and atypical types of mast cells have chloroacetate esterase activity.
已知肥大细胞颗粒的异染程度会因组织固定类型的不同以及不同组织和物种而有所差异。本研究旨在确定犬皮肤中的肥大细胞在固定和染色特性方面是否存在异质性。我们对6只麻醉的特应性犬和1只杂种犬进行了皮肤活检。一份活检组织用福尔马林固定，另一份取自平行腹部部位的活检组织用碱性醋酸铅（莫塔氏溶液）固定。每份活检组织的相邻切片用阿尔辛蓝（1%，pH 0.5）染色或检测氯乙酸酯酶活性。在阿尔辛蓝染色的切片中，福尔马林固定的皮肤中检测到的肥大细胞数量（1,836±454个肥大细胞/mm³，平均值±标准误）比碱性醋酸铅固定的皮肤中少三分之一（2,684±527个肥大细胞/mm³）（P＜0.05）。无论使用何种固定剂，氯乙酸酯酶反应检测到的肥大细胞数量更多。我们得出结论，犬皮肤中存在肥大细胞异质性，这可通过不同类型组织固定后的异染染色来证明。“典型”肥大细胞无论固定与否都能用阿尔辛蓝染色；然而，“非典型”肥大细胞仅在碱性醋酸铅固定后才表现出异染性。典型和非典型类型的肥大细胞都具有氯乙酸酯酶活性。
