Aranega A, Egea J, Alvarez L, Arteaga M
Anat Rec. 1985 Dec;213(4):560-5. doi: 10.1002/ar.1092130412.
The tetralogy of Fallot was produced experimentally in the chick embryo by altering the development of the conus and delaying the disappearance of the conoventricular groove. The experimental procedure used consisted of placing a ligature or cerclage around the proximal portion of the conus for 24 hr in embryos in Hamburger-Hamilton stages 17-21, thus altering cardiac hemodynamics particularly in the clonal region. At the same time the conus was prevented from aquiring its normal position owing to the persistence of the conoventricular groove. As a result, the most frequently observed abnormality (50%) was a complex of anomalies similar to tetralogy of Fallot in man. Varying degrees of pulmonary stenosis were observed in 85% of the experimental cases either as the only defect or in association with other cardiac malformations.
通过改变圆锥部的发育并延迟圆锥室沟的消失，在鸡胚中实验性地制造出法洛四联症。所采用的实验步骤包括在处于汉密尔顿-汉伯格第17至21阶段的胚胎中，在圆锥部近端放置结扎线或环扎24小时，从而改变心脏血流动力学，特别是在克隆区域。与此同时，由于圆锥室沟持续存在，圆锥部无法获得其正常位置。结果，最常观察到的异常情况（50%）是一组类似于人类法洛四联症的异常。在85%的实验病例中观察到不同程度的肺动脉狭窄，要么是唯一的缺陷，要么与其他心脏畸形相关。
